核磁共振法与索氏提取法测定能源植物种子含油量的比较研究

Comparative study on determination of seed oil content of energy plants by using NMR and Soxhlet Extraction

种子含油量是衡量能源植物开发潜力的一个重要指标。为寻找快速、准确测定种子含油量的方法,采用核磁共振法和索氏提取法测定了4种潜在能源植物的种子含油量,并对其进行了分析比较。结果表明:核磁共振法测定的种子含油量明显高于索氏提取法,其中,两种方法测得的曼陀罗种子含油量分别为28.87%和25.91%,海滨锦葵分别为17.74%和14.78%,中国沙棘分别为9.53%和6.82%,蓖麻分别为47.01%和44.31%。核磁共振法测定种子含油量具有简便、无损坏等特点,弥补了索氏提取法耗时,不能提取磷脂、糖脂、脂蛋白等结合态脂类的不足,可用于能源植物种子含油量的快速测定,为筛选潜在能源植物新种质资源提供了可靠方法。

The seed oil content is an important index for evaluating energy plants.To find a quick and precise method for testing the seed oil content,we measured the seed oil contents of four potential energy plants by using the methods of Nuclear Magnetic Resonance（NMR） and Soxhlet Extraction,and compared the results among them.Results showed that the seed oil contents tested by NMR are significantly higher than that of Soxhlet Extraction.For the two methods,the measured seed oil contents of Datura stramonium were 28.87% and 25.91%,respectively;17.74% and 14.78% for Kosteletzkya virginica,respectively;9.53% and 6.82% for Hippophae rhamnoides ssp.sinensis,respectively;and 47.01% and 44.31% for Ricinus communis,respectively.The data indicated that NMR method had the advantages of simple and damage free,which avoided the disadvantages of Soxhlet Extraction such as time-consuming and difficult extraction of fatty acids with combination state（e.g.Lecithin,Glucolipid,Lipoprotein,etc.）.Thus,NMR could be used for rapid testing of the seed oil content,which provides a reliable method for screening potential energy plants.