鸦胆子不同组分抗炎药效伴随毒副作用机制研究

Study on Adjoint Toxical Mechanism of Anti-inflammatory Effect of Different Extract from Fructus Bruceae

目的对鸦胆子水提、醇提组分的抗炎药效伴随毒副作用机制进行研究,为进一步研究其"毒性-功效"相关性提供实验依据。方法建立小鼠巴豆油耳肿胀模型和琼脂肉芽肿模型,并分别给小鼠灌胃高、中、低不同剂量的鸦胆子水提、醇提组分连续3天和7天,末次给药后取血检测血清超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)活性及丙二醛(MDA)、谷胱甘肽(GSH)含量变化。结果连续多次给小鼠灌胃药效剂量的鸦胆子水提和醇提组分,给药后3天仅有高剂量组小鼠血清SOD、GSH-Px活性及GSH含量明显降低,MDA含量明显升高;给药后7天各剂量组小鼠血清SOD、GSH-Px活性及GSH含量均明显降低,MDA含量明显升高,且呈现一定剂量依赖关系。结论过氧化损伤是鸦胆子水提、醇提组分致小鼠抗炎药效伴随毒副作用的重要机制,是否还存在其他作用机制有待进一步研究。

Objective To offer experimental basement for the further study of dependablity between ＂toxicity-efficacy＂ by researching the adjoint toxical mechanism of anti-inflammatory effect of water and alcohol extract from Fructus Bruceae.Methods The model of ear swelling by croton oil and granuloma by agar were made and model mice were administrated with water and alcohol extract from Fructus Bruceae of high,middle and low dosage 3/7 days continually.The activities of serum SOD,GSH-Px and contents of MDA,GSH were detected after the last administration.Results Only activities of SOD and GSH-Px and content of GSH in serum of mice in high dosage group decreased significantly while the content of MDA increased after the third administration of water and alcohol extract from Fructus Bruceae.Serum activities of SOD and GSH-Px and content of GSH of all drug groups decreased significantly while the content of MDA increased after the seventh administration and showes a dependability with dosage.Conclusion Peroxidation injury is a considerable mechanism by which water and alcohol extract from Fructus Bruceae caused the adjoint toxical mechanism of anti-inflammatory effect to mice.Whether there are other mechanisms still need further study.