涎腺肿瘤C-erbB-2癌基因mRNA表达的研究

Expression of C-erbB-2 oncogene mRNA in salivary gland tumors

目的 探讨C erbB 2mRNA表达水平同涎腺肿瘤组织类型、发生和生物学性质的关系。方法 采用Dotblot杂交 ,以32 P标记的寡核苷酸探针 ,以正常涎腺为对照 ,对涎腺肿瘤C erbB 2mRNA表达进行研究。结果 正常涎腺和涎腺肿瘤中发现不同程度C erbB 2mRNA的表达 ,以正常涎腺组织的表达水平为基准 ,在腺淋巴瘤、基底细胞腺瘤呈现低表达、无表达或与正常涎腺组织表达水平相似 ,多形性腺瘤、粘液表皮样癌、腺泡细胞癌、腺样囊性癌、乳头状囊腺癌和肌上皮癌则呈现不同程度的过表达。结论 C erbB 2表达水平同涎腺肿瘤的生物学性质有关。

Objective To study relationship of C erbB 2 oncogene mRNA expression and histotype, tumorigenesis and biological behavior of salivary gland neoplasms Methods Using 32P labeled oligonucleotide as probe, the dot blot technique was used to study the expression of C erbB 2 oncogene mRNA in salivary gland neoplasm with normal salivary gland as control Results With the expression in normal salivary gland as a standard, low C erbB 2 mRNA expression was seen in adenolymphoma, basal cell adenoma However, various degrees of over expression of C erbB 2 oncogene mRNA were detected in pleomorphic adenoma, mucoepidermoid carcinoma,acinic cell carcinoma ,adenoid cystic carcinoma ,papillary cystic carcinoma,and myoethelial cell carcinoma Conclusion C erbB 2 oncogene mRNA over expression in salivary gland neoplasm is related to biological behavior of salivary gland carcinoma