党参多糖的气相色谱-质谱联用分析

Analysis of polysaccharides from Radix Codonopsis by gas chromatography-mass spectrometry

目的:分离纯化党参总多糖并分析单糖组成及其摩尔比。方法:以高效凝胶渗透色谱法检测分离纯化后的党参多糖的分子量;分子量均一的党参多糖经三氟醋酸水解,糖腈乙酰酯衍生化后,利用GC-MS法分析单糖组成,相对校正因子法测定单糖的摩尔比。结果:分离得到5种党参多糖,其中CPS’-3分子量为1.15×105,纯度用面积归一化法计算达到96.43%,由鼠李糖、阿拉伯糖、半乳糖组成,其摩尔比为1.5∶2.7∶4.6;CPS’-4分子量在200万以上,纯度用面积归一化法计算达到99.01%,由鼠李糖、阿拉伯糖、半乳糖组成,其摩尔比为1.3∶0.9∶2.6,均为新发现的党参多糖。结论:党参的2种多糖均由鼠李糖、阿拉伯糖、半乳糖构成,气相-质谱联用技术能够有效地分析党参中的糖类成分。

Objective： To separate polysaccharides from Radix Codonopsis and identify the monosaccharides and their mo- lar ratio. Methods： The molecular weights were tested by High performance gel permeation chromatography.The compo- nents of the polysaccharides were identified by gas chromatography-mass spectrometry （GC-MS）. Monosaceharides from polysaccharides were derivatized to acetylated aldononitriles before determination. Results： Polysaccharide were composed of CPS＇-3 and CPS＇-4, all including rhamnose, arabinose and galactose, with the molar ratio of 1.5：2.7：4.6, 1.3：0.9：2.6 in different CPS.They were newly discovered polysaccharides in Radix Codonopsis. Conclusion： CPS were made up of rham- nose, arabinose and galactose.The GC-MS was eligibled for the analysis of Radix Codonopsis polysaccharides.