细胞培养三种染色法检测沙眼衣原体

Detection of chlamydia trachomatis infection by three staining methods of cell culture

用细胞培养法对１６０ 例有淫乱史病人标本进行沙眼衣原体培养，后经免疫荧光法，Ｇｉｅｍ ｓａ 染色法和碘染法鉴定，结果表明， 免疫荧光法的敏感度最高 （４８／１６０， ３００％ ）， Ｇｉｅｍ ｓａ法次之 （４３／１６０， ２６９％ ）， 碘染色法较低 （３９／１６０， ２４４％ ），但后两者简便快速， 可以节省昂贵的试剂购置费用， 仍是有待发展的检测技术； 沙眼衣原体标本贮存以液氮最佳，在７ 天内，－ ７０°Ｃ是一个临界分离温度。

To evaluate three staining methods of cell culture for detection of chlamydia trachomatis in 160 clinical specimens from patients in a sexually transmitted disease.These results showed that positive rate of detection with monoclonal antibody immunofluorescent technique,Giemsa staining and iodine staining were 30 0%,26 9% and 24 4% respectively.Of the two the latters possessed simple,cheap and rapid.Positive rate decrease 6 7% when specimen stored at -70 °C .