摘要
用细胞培养法对160 例有淫乱史病人标本进行沙眼衣原体培养,后经免疫荧光法,Giem sa 染色法和碘染法鉴定,结果表明, 免疫荧光法的敏感度最高 (48/160, 300% ), Giem sa法次之 (43/160, 269% ), 碘染色法较低 (39/160, 244% ),但后两者简便快速, 可以节省昂贵的试剂购置费用, 仍是有待发展的检测技术; 沙眼衣原体标本贮存以液氮最佳,在7 天内,- 70°C是一个临界分离温度。
To evaluate three staining methods of cell culture for detection of chlamydia trachomatis in 160 clinical specimens from patients in a sexually transmitted disease.These results showed that positive rate of detection with monoclonal antibody immunofluorescent technique,Giemsa staining and iodine staining were 30 0%,26 9% and 24 4% respectively.Of the two the latters possessed simple,cheap and rapid.Positive rate decrease 6 7% when specimen stored at -70 °C .
出处
《临床检验杂志》
CAS
CSCD
北大核心
1999年第5期278-279,共2页
Chinese Journal of Clinical Laboratory Science