摘要
于培养的新生大鼠海马神经细胞，应用ｆｕｒａ２ 荧光检测技术，观察了致疒间剂马桑内酯对单个海马神经细胞内钙离子浓度（［Ｃａ２＋ ］ｉ）的影响。培养液内马桑内酯浓度达１０－ ８ｍ ｏｌ／Ｌ时可引起［Ｃａ２＋ ］ｉ增加，［Ｃａ２＋ ］ｉ随给药浓度的提高而升高。马桑内酯浓度达５×１０－ ８ｍ ｏｌ／Ｌ时，［Ｃａ２＋ ］ｉ维持于稳定的高水平上。当胞外无Ｃａ２＋时，马桑内酯仍可引起［Ｃａ２＋ ］ｉ的升高；Ｌ型钙通道阻断剂ｖｅｒａｐａｍ ｉｌ不能阻断马桑内酯的上述作用。结果提示，海马神经细胞在致疒间剂作用下，发生Ｃａ２＋ 内流及胞内钙池释放现象。
The effect of convulsive agent coriaria lactone (CL) on intracellular free calcium concentration ([Ca 2+ ]i) of cultural hippocampal single neuron was observed by using fura 2 microspectroflurometric technique.10 -8 mol/L CL could cause increase of [Ca 2+ ]i in single neuron of hippocampus. When CL was raised to 5×10 -8 mol/L,[Ca 2+ ]i of neuron was maintained at a higher steady level. An increase of [Ca 2+ ]i also could be induced by CL in Ca 2+ free medium. L calcium channel blocker verapamil could not completely block the effect of CL. The results suggest that chemical convulsive agent CL could cause the influx of extracellular Ca 2+ and the release of Ca 2+ from intracellular calcium pool in hippocampus neuron.
基金
国家自然科学基金
