乳腺肿瘤患者血清Cu Zn Fe含量与细胞增殖的关系

The relation between serum copper,zinc and iron levels and cell proliferation in patients with breast tumor

目的：观察良、恶性乳腺疾病 Ｃｕ、 Ｚｎ、 Ｆｅ 及 Ｃｕ／ Ｚｎ 比值的变化，以及探索这些微量元素与 Ｃ－ｅｒｂ Ｂ－ ２、ｐ５３、 Ｋｉ－ ６７ 之间可能存在的关系。方法：对４２ 例浸润性导管癌、４５ 例纤维腺瘤和小叶增生的患者进行了 Ｃｕ、 Ｚｎ、 Ｆｅ 含量（火焰原子吸收法）及 Ｋｉ－ ６７、 Ｃ－ ｅｒｂ Ｂ－ ２、ｐ５３ 基因产物（免疫细胞化学法）的分析。结果：良、恶性乳腺肿瘤之间， Ｃｕ、 Ｆｅ 含量及 Ｃｕ／ Ｚｎ 比值均有显著性差异（ Ｐ＜ ０．０２； Ｐ＜ ０．０２； Ｐ＜０．００１）， Ｚｎ 值无显著性差异（ Ｐ＞ ００５）。乳腺疾病中 Ｇ－ ｅｒｂ Ｂ－ ２ 阳性与否均与 Ｃｕ、 Ｚｎ、 Ｆｅ 含量有显著性差异（ Ｐ＜ ００２； Ｐ＜ ０００５； Ｐ＜ ００５），且 Ｃ－ ｅｒｂ Ｂ－ ２ 阳性时， Ｃｕ、 Ｚｎ、 Ｆｅ 含量比其阴性时高。结论： Ｃｕ、 Ｚｎ、 Ｆｅ 含量变化与 Ｃ－ ｅｒｂ Ｂ－ ２ 基因增强表达、肿瘤恶变密切相关。

In order to observe the variance of copper,zinc and iron levels and Cu/Zn ratio on 42 benign and 45 malignant breast tumors,especially the relation between the results of C-erbB-2,P53,Ki-67 and of trace elements,we determined copper,zinc and iron by atomic absorption spectrophotometer,detected the expressions of Ki-67,P53 and C-erbB-2 with immunocytochemistry methods.The results showed that between benign and malignant breast tumors,copper and iron was different P<0.02and Cu/Zn ratio was significantly different(P<0 001),but zinc was no difference.In addition,copper,zinc and iron levels were higher in C-erbB-2 protein product positive cases than that in C-erbB-2 negative cases of breast tumor including the benign and malignant (P<0 02;P<0.005;P<0.05).So,maybe we can draw a conclusion from the above that copper,zinc and iron play an important role in the over expression of C-erbB-2and growth of the tumor.