5-氟尿嘧啶加奥曲肽对乳腺癌细胞凋亡的影响

The effect of sandostatin and 5 fluorouracil on apoptosis in breast cancer

目的 探讨５氟尿嘧啶（５ＦＵ）加奥曲肽（ 善得定） 对乳腺癌细胞凋亡的影响。 方法 取手术切除的１６ 例乳腺癌标本，制备癌单细胞悬液。每例癌细胞均分成４ 组：５ＦＵ组（ 加入５ＦＵ１０μｇ／ｍｌ）、善得定组（ 加入善得定０－１ μｇ／ｍｌ） 、５ＦＵ加善得定组（ 加入５ＦＵ１０ μｇ／ｍｌ、善得定０－１ μｇ／ｍｌ）及对照组（不加任何药物） 。用ＤＮＡ断端标记法（ＴＤＴ法） 检测乳腺癌细胞凋亡率。 结果 １６ 例标本加入相应药物１８ 小时后，５ＦＵ组凋亡率为（２１－８±６－６）％ ，善得定组为（１４－３±８－８）％ ，５ＦＵ 加善得定组为（２６－７±６－９）％ ；当按雌激素受体（ＥＲ） 、孕激素受体（ＰＲ） 是否阳性分成两组时，ＥＲ、ＰＲ阳性（ ＋ ）组５ＦＵ加用善得定后凋亡率为（３２－２±７－９） ％ ，而ＥＲ、ＰＲ阴性（ －） 组则为（２０－５ ±５－５） ％ ，两组相比差异有显著意义（Ｐ＜０－０５） 。 结论 ５ＦＵ加用善得定后对ＥＲ、ＰＲ（ ＋） 的乳腺癌细胞较ＥＲ、ＰＲ（ － ）的癌细胞为敏感，故在ＥＲ、ＰＲ（ ＋） 的乳腺癌患者中应用较为理想。

Objective To investigate the effect of 5 FU plus sandostatin on apoptosis in breast cancer. Methods Breast cancer samples from 16 patients were treated with 5 FU (10 μg/ml) and sandostatin (0 1 μg/ml) in vitro using the in situ terminal deoxynucleotidyl transferase assay (TDT) to detect apoptosis. The patients were female (mean age 54 4 years). In the 16 patients, 10 had positive estrogen receptor (ER) and positive progestogen receptor (PR), and the rest had negative ER and PR. Results 18 hours after addition of 5 FU, sandostatin and both, the apoptotic rate of tumor cells was (21 8±6 6)%,(14 3±8 8)%, and (26 7±6 9)% respectively. When samples were divided into 2 groups according to the sensitivity of ER and PR, the apoptotic rate of 5 FU plus sandostatin was (32 2±7 9)%,in positive ER and PR group, but (20 5±5 5)% in negative ER and PR group( P <0 05). Conclusions 5 FU plus sandostatin is more sensitive in the positive ER and PR group than in the negative ER and PR group. 5 FU plus sandostatin would be helpful in the biotherapy of breast cancer partients with positive ER and PR.