节段型白癜风皮损区角朊细胞表达干细胞因子的实验研究

Study of SCF expression in keratonicytes of segmental vitiligo lesions

目的：了解节段型白癜风皮损区角朊细胞（ＫＣ）表达干细胞因子（ＳＣＦ）的状况。方法：采用原位杂交及免疫组化技术对８例活动期和９ 例稳定期节段型白癜风患者皮损ＫＣ内ＳＣＦ的表达进行了观察。结果：活动期皮损ＫＣ表达ＳＣＦ明显增强，稳定期皮损与正常皮肤ＫＣ的表达状况无显著差异。结论：稳定期皮损ＫＣ内ＳＣＦ的正常表达为黑素细胞（ＭＣ）自体移植治疗节段型白癜风，移植ＭＣ能够长期存活提供了一定的实验依据，活动期ＫＣ内ＳＣＦ表达增强提示ＳＣＦ可能参与了该型白癜风的发病。

Objective: To study the expression of stem cell factor (SCF) in the keratinocytes (KCs) in the lesions of segmental vitiligo. Methods: In situ hybridization with a human SCF cDNA probe and immunohistochemistry were employed to study the lesions of 8 active and 9 stable cases of segmental vitiligo. Results: It was found that the expression of SCF in the KCs of the lesions of segmental vitiligo was more intense in the active stage of the disease than in the stable stage and no significant difference was found between the expressions in the normal KCs and the stable KCs. Conclusion: Normal expression of SCF in the stable lesions of segmental vitiligo provides experimental evidence of longtime survival of autotransplanted melanocytes when they are used to treat segmental vitiligo. The intense expression of SCF in KCs in the active stage indicates the participation of SCF in the pathogenesis of the disease.