人参皂甙单体Rb1对多药耐药细胞系K562/HHT的耐药逆转作用

The reverse effects of Ginseng Saponin Rb1 on multidrug resistance of human leukemic cell line K562/HHT iv vitro

目的：探讨中药钙离子通道拮抗剂人参皂甙单体Ｒｂ１，对白血病多药耐药细胞系（Ｋ－５６２／ＨＨＴ）的耐药逆转作用。方法：药物敏感试验采用四唑氮盐（ＭＴＴ）比色试验法。结果：Ｒｂ１在０～８０ μｍ ｏｌ／Ｌ浓度范围内对Ｋ５６２／ＨＨＴ未见明显毒性作用；２０ μｍ ｏｌ／Ｌ、４０ μｍ ｏｌ／ＬＲｂ１ 耐药逆转倍数分别为４．４、７．９ 倍。结论：Ｒｂ１ 可以逆转Ｋ５６２／ＨＨＴ的多药耐药性。

Objective: To study the reverse effects of Ginseng Saponin Rb1 on the multi drug resistance of human leukemic cell line K562/HHT in vitro . Methods: Cytotoxic effects were determined with MTT colorimetry. Results: Saponin Rb1 in the concentration range of 0～80 μmol/L showed notoxic effects on the cells of human leukemic cell line K562/HHT and when the concentration was increased to 20 μmol/L and 40 μmol/L, the reverse effects on the multidrug resistance of the leukemic cells were increased by 4.4 and 7.9 fold respectively. Conclusion: Ginseng Saponin Rb1 is able to reverse the multidrug resistance of the cells of human leukemic cell line K562/HHT in a dosage dependent manner in vitro.