pSV PoM cat微基因修饰雪旺细胞脊髓内移植对损伤脊髓细胞的保护作用

Protective effects of intracord transplantation of pSVPoMcat modified Schwann cells on spinal cord injury

目的：观察ｐＳＶＰｏＭｃａｔ微基因修饰雪旺氏细胞（ＳＣ）脊髓内移植对损伤组织的作用的影响。方法：将脊髓半横断损伤ＳＤ大鼠模型随机分为ｐＳＶＰｏＭｃａｔ微基因修饰ＳＣ移植组（Ａ组）、ＳＣ移植组（Ｂ组）、损伤对照组（Ｃ组）和正常对照组（Ｄ组）。８ ｈ 后一半动物取伤段脊髓标本测水离子含量。另一半动物采用联合行为记分（ＣＢＳ）评价其神经功能。结果：脊髓损伤（ＳＣＩ）后组织水肿，Ｎａ＋ 、Ｃａ２＋ 离子浓度升高，Ｋ＋ 、Ｍｇ２＋ 离子浓度降低，ｐＳＶＰｏＭｃａｔ微基因修饰ＳＣ脊髓内移植可显著改善这些变化，且使ＳＣＩ后神经功能有显著改善。结论：ｐＳＶＰｏＭｃａｔ微基因修饰ＳＣ脊髓内移植对ＳＣＩ具有保护作用，其机制可能与减少神经细胞离子失衡，改善细胞内环境有关。

Objective: To study the protective effects of the intracord transplantation of microgen pSVPoMcat genetically modified Schwann cells (MSCs) on spinal cord injury (SCI). Methods: Rats with semi division (SD) of the spinal cord was divided into 4 groups. Group A consisted of the rats with SD treated with the transplantation of MSCs, Group B of the rats with SD treated with the transplantationof SCs without genetic modification, Group C of the rats with SD without treatment and Group D was the normal control. 8 h after operation, half of the rats of each group were killed and the injured segment of the spinal cord was resected to be examined with atomic absorption spectrophotometry. Another half of the rats of all the groups were examined with neurological function tests to have a combined behavioral score (CBS). Results: There was a significant increase of water content and Na + and Ca 2+ ions and a decrease of K + and Mg 2+ ions in the injured cord segment of Group C and a statistically significant recovery was obserbed in Group A. The intracord transplantation of pSVPoMcat genetically modified SCs improved the neurological outcome of spinal cord injury. Conclusion: Our findings indicate that intracord transplantation of pSVPoMcat genetically modified Schwann cells exerts protective effects on the injured segment of the spinal cord through the improvement of the internal ion environment of the spinal cord.