免疫酶斑点法(IEDA)检测白色念珠菌芽管特异性抗原的临床研究

Clinical observation of detecting germ tube specific antigen of Candid alblicans by IEDA

目的：试图寻求一种较理想的系统性念珠菌病的血清学诊断方法。方法：制备白色念珠菌芽管特异性抗体，采用ＩＥＤＡ对临床病人血清中的相应抗原进行检测。结果：①检测的最低敏感浓度为０．２５ μｇ／ｍ ｌ；②以１４ 例系统性念珠菌病患者为实验组，以１５ 例浅表白念感染者，１１ 例其它疾病患者及２０位正常人为对照组，检测的敏感性为７１．４％ ，特异性为１００％ 。结论：该方法敏感性较高，特异性强，且具有简便经济的特点。有助于系统性念珠菌病的诊断，尤其适宜在基层医院普及应用。

Objective: To find a better serologic diagnostic method for systemic candidiasis. Methods: Germ tube specific antibody of Candida albicans was prepared and used to detect corresponding antigen in serum of patients by IEDA. Results: ①The lowest sensitive concentration was 0.25 ng/ml; ②The sensitivity of this method was 71.4%. There was no germ tube specific antigen detected in sera of 15 patients with superficial candidiasis, 11 with other diseases and 20 normal controls. The specificity was 100%. Conclusion: This method has high sensitivity and the method is simple. So IEDA can be helpful to the diagnosis of systemic candidiasis.