人胰腺癌细胞nm23mRNA的表达与其转移潜能的关系

Relationship between the expression of nm23 gene mRNA in the cell line of human pancreatic cancer and its metastatic potential

目的 研究人胰腺癌细胞中ｎｍ２３ ｍＲＮＡ的表达与其转移潜能的关系。 方法 在建立不同转移潜能的人胰腺癌ＪＦ３０５ 单克隆细胞亚系的基础上，应用定量逆转录多聚酶链式反应技术（ＲＴＰＣＲ）检测不同转移潜能的人胰腺癌ＪＦ３０５ 单克隆细胞亚系细胞中ｎｍ２３ ｍＲＮＡ 的表达。 结果 ｎｍ２３Ｈ１ 基因ｍＲＮＡ在低转移潜能的ＪＦ３０５Ｃ细胞系中的表达（１－９０ ±０－０９）明显高于其在高转移潜能的ＪＦ３０５Ａ 细胞系中的表达（１－６３±０－１０，Ｐ＜ ０－０１）；ｎｍ２３Ｈ２ 基因ｍＲＮＡ的表达，在高、低转移潜能的细胞亚系间差异无显著意义（０－７９ ±０－１０ ，０－７７±０－０６，Ｐ＞０－０５）。 结论 ｎｍ２３ 基因，主要是ｎｍ２３Ｈ１ 基因。

Objective To study the relationship between the expression of nm23 gene mRNA in the cell line of human pancreatic cancer and its metastatic potential. Methods Using the quantitative reverse transcription PCR procedure,we observed the expression of nm23 mRNA in human pancreatic cancer JF305 cell sublines with different metastatic potential. Results The nm23 H1 gene mRNA level in low metastatic potential cell subline JF305C (1 90±0 09) was higher than that in high metastatic potential cell subline JF305A(1 63±0 10, P <0 01). The differences of nm23 H 2 gene mRNA levels between the two different metastatic potential sublines were not statistically significant (0 79±0 10;0 77±0 06. P >0 05).Conclusion It is the nm23 H 1 gene not,nm23 H 2 gene that plays a suppressive role in the metastasis in human pancreatic cancer JF305 cell sublines.