摘要
目的:研究模拟混合何首乌中7 种恩醌类成分的分离。方法:添加有机溶剂的胶囊电动毛细管色谱法。结果:大黄素苷、大黄素、芦荟大黄素、大黄酸、大黄素甲醚苷、大黄酚和大黄素甲醚在25mmol/L硼砂含25mmol/LSDS和10% 乙腈(pH9-2) 的缓冲溶液中16min 内得到完全分离。结论:添加有机溶剂的胶囊电动毛细管色谱法对恩醌类成分的分离具有明显的优越性。
OBJECTIVE:To study the separation of mimic-mixed 7 kinds of antraquinone derivatives.METHODS:Micellar electrokinetic capillary chromatography with additive of organic solvent was used.RESULTS:Emodin,chrysophanl,physcion,rhein,emodin-8-o-β-D-glucopyranoside,aloe-emodin,and Physcion-8-o-β-glucopyranoside were separated successfully in 16 min in the buffer of 25 mmol/L H 3BO 3(pH9.2)containing 25mmol/L SDS and 10% acetonitrile.CONCLUSION:Micellar electrokinetic capillary chromatography with additive of organic solvent has potential advantages on separation of anthraquinone derivatives.
出处
《药学实践杂志》
CAS
1999年第6期352-354,共3页
Journal of Pharmaceutical Practice
关键词
胶囊电动毛细管
色谱
何首乌
分离
蒽醌
micellar electrokinetic capillary chromatography, polygonum multiflorum thunb,separation