黄连生物碱在小鼠胃粘膜的动态分布

Dynamic distributing of alkaloids of Rhizoma Coptidis in gastric munosa in mice

目的：探讨连香胶囊主要成分黄连生物碱的小鼠胃粘膜的动态分布规律。方法：采用薄层色谱法鉴定小鼠给药后胃粘膜中的生物碱成分，并用紫外分光光度法定量测定不同剂量、不同给药时间胃粘膜的生物碱含量。结果：给药后１５ ｍｉｎ，胃粘膜生物碱浓度达到高峰，之后缓慢降低。给药剂量在４００ ｍｇ·ｋｇ－ １ 以下时，胃粘膜吸附药量随给药剂量的增大而增加，两者之间有良好的线性关系（Ｐ＜ ０．０１），当给药剂量大于４００ ｍｇ·ｋｇ－ １ 时，胃粘膜吸附药量不再增加。按一级动力学计算，生物碱在胃粘膜的半衰期Ｔ１／２ 为１９４ ｍｉｎ。结论：黄连生物碱在小鼠胃粘膜吸收迅速，清除则较慢，生物碱的吸附量与给药剂量在５０～４００ ｍｇ·ｋｇ－１ 范围内有良好的正相关性。

OBJECTIVE:To explore rules of absorption and elimination of alkaloids in gastric munosa in mice.METHODS:To take mice,administrate with Liangxiang Capsule,take stomach,purify alkaloids with 0.05 mol·L -1 sulfuric acid solution,then identify alkaloids of Rhizoma Coptidis by thin layer chromatography and determine alkaloids by ultraviolet spectrophotometry.RESULTS:The results showed that concentration of alkaloids had achieved the highest value at administration 15 minutes later,but it was eliminated slowly.The concentration increased with dosages of Liangxiang capsule,a good relationship was shown between concentration and dosage that was less than 400 mg·kg -1 ( P < 0.05 ).CONCLUSION:Alkaloids of Rhizoma Coptidis was absorbed quickly and was eliminated slowly in gastric munosa.There is a direct ratio relationship between concentration of alkaloids and dosage of Liangxiang Capsule in the range from 50 to 400 mg·kg -1 .