摘要
目的:测定通脉活络丸中黄芪甲苷的含量。方法:用甲醇边提取边皂化,然后用氯仿正丁醇(2∶1) 萃取等步骤制成供试品,以薄层扫描法测定,λS=530 nm ,λR= 680 nm 。结果:平均回收率为97.47% ,RSD=2 .42 % ,两批样品中黄芪甲苷的含量分别为0 .038 9 % 和0.041 5% 。结论:该方法较为简便,经济。
OBJECTIVE:To determine astragaloside A in Tongmai Huoluo pills.METHODS: Tongmai Huoluo pills were extracted and saponificated with methyl alcohol simultaneously,and then were extracted and processed into the samples with chloroform butanal(2∶1) and so on,finally the samples were determinated by TLC scanning(λ S=530 nm,λ R=680 nm).RESULTS:Average recovery was 97.47% ,and RSD = 2.42% .The contents of the astragaloside A in two groups of samples are 0.038 9% and 0.041 5% respectively.CONCLUSIONS:The method is simple,convenient and economical.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
1999年第11期667-668,共2页
Chinese Journal of Hospital Pharmacy
关键词
薄层扫描法
通脉活络丸
含量测定
黄芪甲苷
TLC scanning, Tongmai Huoluo pills,determination,astragaloside A