肉鸡热应激下肝脏和下丘脑HSP70 mRNA的表达

Expression of HSP70 mRNA in liver and hypothalamus in chicken under heat stress

研究优质黄羽肉鸡热应激期间肝脏和下丘脑组织中热休克蛋白70(heat shock protein 70,HSP70)基因mRNA的动态表达规律,为揭示该类型优质鸡种热应激反应机理提供参考。64日龄Anak 40(♂)×岭南黄(♀)杂交后代母鸡置于(38±1)℃恒温室内进行持续热应激处理,采取荧光定量PCR方法分别测定热应激起始(0 h)、热应激后12、24、36、48和60 h肝脏和下丘脑组织中HSP70 mRNA的表达水平。随着热应激时间的延长,肝脏和下丘脑组织中HSP70 mRNA表达水平均显著上升,至热应激36 h时表达水平达高峰值,约为应激起始表达水平的7倍,差异显著(P<0.05);热应激48和60 h,下丘脑HSP70 mRNA表达量快速回落至热应激初期的水平,而肝脏表达量极显著地骤降至低于热应激初期10%的水平。在(38±1)℃持续热应激期间,优质肉鸡肝脏和下丘脑HSP70 mRNA表达呈现基本同步的变化规律,即表达水平先上升后下降,热应激36 h时表达水平达高峰值。

This study is to investigate the dynamic expression profiles of HSP70mRNA in liver and hypothalamus in quality chicken under heat stress,and explore the mechanism of heat stress reaction of this kind of chicken.The 64-day-old subjects produced from Anak 40（♂） × Lingnan Yellow（♀） were placed in a room with the ambient temperature at（38±1）℃ for heat stress treatment.The HSP70mRNA levels in liver and hypothalamus were detected by fluorescence quantitative PCR at the initial,12,24,36,48,and 60 h of the stress,respectively.During heating stress,the expression of HSP70mRNA in the liver and hypothalamus markedly increased,and reached the peak at 36 h,which significantly（P0.05） higher than the initial level by approximately 7-fold.At 48 and 60 h of the stress,the HSP70mRNA in hypothalamus decreased dramatically to the level as at the initial of heat stress.While in liver,the HSP70mRNA significantly decreased to the level less than 10% of the initial.Under heat stress of（38±1）℃,the expression of HSP70mRNA in the liver and hypothalamus increased up to 36 h and followed by a sharp decrease,and the dynamic expression profiles of HSP70mRNA in both tissues were basically synchronous.