蛔虫变应原致喘豚鼠后外周血血液流变性的动态变化

DYNAMIC CHANGES OF HEMORREOLOGY AT DIFFERENT TIMES OF THE ASTHMA MODEL OF THE GUINEA PIGS IN IMMUNIZED AND CHALLENGED WITH ASCARIS ALLERGEN

目的：观察蛔虫变应原致喘豚鼠动物模型的血液流变学变化的动态变化、丰富变应原激发动物哮喘的内容。方法：把实验豚鼠随机分为阴性对照组、佐剂对照组和阳性激发组。用蛔虫变应原激发致喘豚鼠后，观察各实验组在激发哮喘后１小时、２４小时和７２小时的血液流变学变化。结果：由蛔虫变应原引起的过敏性哮喘豚鼠动物模型，其血液流变性出现异常，阳性激发组的血浆粘度分别为６，６２±０．２８，５１.４± ０．１１，４．８６± ０．２３，远高于阴性对照组（平均为 ４．５７± ０．２０）和佐剂对照组（平均为 ４．６３±０．１７），有显著性意义（Ｐ＜０．０１）；红细胞聚集指数和全血高切还原粘度也均较阴性对照组、佐剂对照组显著性升高（Ｐ＜０.０１）；而红细胞压积则无明显变化。结论：用蛔虫变应原致喘豚鼠后，血液出现高粘滞状态，进一步加剧气道局部的缺血、缺氧及炎性反应，导致气体交换障碍、肺功能降低。

In order to observe the dynamic changes of hemorreology at different times of the asthma model of the guinea pigs immunized and challenged with Ascaris allergen, and to enrich the theoryof the asthma models, Guinea pigs were divided into three groups( negative control group, adjuvant control group, and positive control group ). After guinea pigs were challenged with Ascaris allergen, the dynamic changes of hemorreology at different times after challenge were also examined. The results suggested that there existed abnormal situation in hemorreology. In positive challenge group, the viscosity of blood plasma were 6.62 ?0.28,5.14 ?0.11,4.86 ?0.23 after challenged 1h,24h and72h,and there ex-isted significant difference compared with negative control group(4. 57 ?.20) and adjuvant control group (4.63 ?.17) .And the gathering index of red cell and reduction viscosity of blood increased sig-nificantly, compared with those in negative control group and adjuvant control group(P < 0.01). But there're n o differences in hematocrit in three groups. These results suggested that high conglutination could be induced in Ascaris asthma model after challenged by Ascaris allergen, which could facilitate allergic reaction in airway.