大鼠骨髓间充质干细胞的体外分离与原代培养

In vitro isolation and primary culture of bone marrow mesenchymal stem cells

目的探讨大鼠骨髓间充质干细胞(bone marrow mesenchymal stem cells,BMSCs)的体外分离与原代培养过程中应注意的问题。方法全骨髓贴壁培养筛选法分离和培养大鼠BMSCs,通过不断换液及传代纯化培养的细胞。结果大鼠BMSCs传至P3代后,形态为均匀分布的梭形成纤维样细胞。结论全骨髓贴壁培养筛选法操作简便,需要的骨髓量少,原代培养时更符合细胞生长的微环境,是培养大鼠BMSCs的理想方案。

【Objective】To investigate the problem of In vitro isolation and primary culture of bone marrow mesenchymal stem cells.【Methods】Adherent culture and isolation method of whole bone marrow was employed to acquire BMSCs,and the cultured BMSCs were purified by continuous change of medium and pass on from generation to generation.【Results】P3 generation of rat BMSCs demonstrated uniform distribution of the spindle or fiber-like cells.【Conclusion】The whole bone marrow adherent culture method,which had the advantage of simple,required less bone marrow,and accorded with the micro-environment of cell growth,is the ideal solution for rat BMSCs culture.