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杨梅黄酮减弱MPP^+诱导的MES23.5细胞的细胞毒性(英文)

Myricetin Attenuates MPP^+-induced Cytotoxicity in MES23.5 Cells
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摘要 目的:探讨杨梅黄酮对MPP+处理的MES23.5细胞的保护作用。方法:实验分为空白对照组,MPP+处理组,MPP+和杨梅黄酮共处理组,分别处理MES23.5细胞24h后,应用Hoechst 33258染色观察各组细胞细胞核形态的改变,应用RT-PCR技术观察Bcl-2和Bax mRNA表达的改变。结果:杨梅黄酮能明显抑制MPP+引起的MES23.5细胞核固缩,核碎裂等形态学的改变,杨梅黄酮还可以对抗MPP+处理造成的MES23.5细胞的Bcl-2/Bax比率的降低。结论:杨梅黄酮对MPP+处理的MES23.5细胞具有保护作用。 To investigate the neuroprotective effect of myricetin on 1-methyl-4-phenylpyridinium （MPP^＋）-treated MES23.5 cells. Methods： MES23.5 cells were treated with vehicle, MPP^＋, MPP^＋ and myricetin for 24h respectively. By Hoechst staining and RT-PCR, the nuclear morphological changes and the Bel-2 and Bax mRNA levels were investigated. Results： The results showed that myricetin treatment significantly attenuate MPP^＋-induced cell apoptosis. MPP^＋ treatment induced decrease of Bcl-2/Bax mRNA ratio was also inhibited by myricetin. Conclusion： Myricetin may protect against MPP^＋-induced cytotoxicity in MES23.5 cells.
作者 张凯 马泽刚 王俊 谢安木
机构地区 国家生理学重点(培育)学科山东省神经相关疾病的机制与防治重点实验室 青岛大学医学院生理学教研室 青岛大学医学院附属医院神经内科
出处 《现代生物医学进展》 CAS 2011年第10期1830-1833,共4页 Progress in Modern Biomedicine
基金 Natural Science Foundation of China(30800353) the Bureau of Science and Technology of Qingdao(09-1-1-29-nsh)~~
关键词 杨梅黄酮 MPP^+ 凋亡 MES23.5 CELLS Myrieetin MPP^＋ Apoptosis MES23.5 cells
分类号 R285.5 [医药卫生—中药学] Q813 [生物学—生物工程]
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现代生物医学进展
2011年 第10期

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