生半夏、南星水提物对人胃癌BGC823细胞的侵袭力及HIF-1α mRNA蛋白表达的影响

Effects of Aqueous Extract of Chinese Medicine Raw Pinellia and Nanxing on Human Gastric Cancer Cells BGC823

目的:观察生半夏、南星中药水提物对缺氧环境中人胃癌细胞株BGC823细胞HIF-1α蛋白表达和侵袭力的影响。方法:运用CoCl(2氯化钴)诱导BCG823细胞缺氧,使得细胞中HIF-1α蛋白表达升高.实验组加入生半夏、南星水提物对细胞进行预处理,然后在进行缺氧诱导。通过甲基噻唑基四唑法(MTT)检测细胞活性,使用Transwell检测细胞侵袭能力变化,RT-PCR、Western blotting分别检测HIF-1α mRNA及蛋白含量及变化。结果:生半夏、南星水提物能抑制人胃癌BGC823细胞的增殖;生半夏、南星水提物均能抑制缺氧诱导胃癌细胞的侵袭力,并且能降低HIF-1α mRNA及蛋白表达。结论:生半夏、南星水提物可抑制人胃癌BGC823细胞的增殖,抑制人胃癌BGC823细胞侵袭力,可能通过降低HIF-1α蛋白表达有关。

To investigate the effects of the enthanol and nanxing extract from Rhizome Pinelliae Preparata on hypoxiainducible -1 alpha protein expression and aggressive influence in human gastric cancer cells BGC823. Methods： COC12 （chlorinated cobalt） was used to induce BCG823 cytopathic hypoxia approach, which leads the increasing the expression of hypoxia-inducible -1 alpha. The cell viability was detected by MTT chromatometry and cell growth curve was generated; Transwell small room was used to detect cells attack ability, RT-PCR, Western blotting was use to detect hypoxia-inducible-l alpha mRNA expression and protein level. Results The extraction of enthanol and nanxing can inhibit the proliferation of cells BGC823 human cancer;The enthanol and nanxing extrac can inhibit the aggressive ability of cells BGC823 human cancer cells, the cause of which may be the decreasing of the expression of HIF-1 alpha mRNA and protein. Conclusion： Enthanol extract from Rhizome Pinelliae Preparata can inhibit the BGC823 cells proliferation and Restrain BGC823 human cancer cells aggressie influence by lowering hypoxia-inducible - 1 alpha mRNAand the protein exoression.