人体小脑神经元发育的定量观察

Quantitative Observation on Development of Human Cerebellar Neurons

目的:研究人体小脑神经元的发育过程。方法:应用体视学方法,对18例不同时期人体小脑组织Golgi染色后进行观察,观测小脑皮质分层出现的时间,观测并计算神经元的数密度、体密度和表面积密度。结果:6月龄时,小脑皮质出现较明显的分子层、蒲肯野细胞层和颗粒层;星形细胞、篮状细胞、蒲肯野细胞、颗粒细胞和高尔基细胞的的数密度随月龄/年龄的增长而减少,体密度和表面积密度随月龄/年龄的增长而增加,但这些减小和增大是不等速的,6-8月龄变化最明显。结论:人体小脑神经元的发育呈现快慢交替、不均速发展,6～8月是小脑神经元发育的重要时期。

To investigate＇the development progrss of cerebellar neurons in human body. Methods： Eighteen differentperiods cerebellar neurons were observed after Golgi staining on layered appearance time of the cerebellar cortex and number density, bulk density ＆ surface area density of neurons by stereology.Results：In the sixth moon＇s age, apparent molecular layer, Purkinje cell layer and granular layer were observed in cerebellar cortex. The number densities of stellate cells, basket cells, purkinje cells, granule cells and golgi cells decreased by the increment of month/age, while bulk densities and surface area densities increased. But these decrease and increase were not constant, with the most apparent changes from the sixth to the eighth moon＇s ages. Conclusion：The development of human cerebellar neurons shows fasting-slowing alternation and uneven speed.The moon＇s ages from the sixth to the eighth are the important periods of development of cerebellar neurons.