白香丹胶囊干预经前期综合征肝气逆证大鼠血清代谢组学研究

Metabonomics study on effect of Baixiangdan Capsule on premenstrual syndrome with liver-qi invasion in rats

目的通过代谢组学方法研究白香丹胶囊(白芍、香附和丹皮)干预经前期综合征肝气逆证大鼠血清内源性代谢物的变化,探索与该病证密切相关的代谢模式及白香丹胶囊的作用途径。方法基于代谢组学理论,以UPLC/Q-TOF-MS为技术平台,建立大鼠血清代谢指纹谱,通过多元统计分析方法研究PMS肝气逆证大鼠血清的代谢差异及白香丹的干预作用。结果模型组、给药组与对照组的血清内源性代谢物发生了明显变化,模型组血清中肾上腺素、四氢脱氧皮质酮、5a-四氢皮质醇、雌酚酮、赖氨酸、5-羟基赖氨酸、乙酰半胱氨酸水平升高,孕二醇水平降低,给药组中显示白香丹胶囊对其有调节作用。白香丹表现出糖皮质激素、雌激素、神经递质及氨基酸等多靶点的作用特点。结论应用代谢组学方法可以从整体出发研究PMS肝气逆证及中药复方干预后的代谢模式差异,为PMS的临床诊断和治疗提供依据。

AIM To study Baixiangdan Capsule（Paeoniae radix alba,Cyperi Rhizome,Moutan Cortex） on the change of endogenous metabolites in premenstrual syndrome with liver-qi invasion in rats.METHODS A UPLC/Q-TOF-MS method was developed for serum metabonome analysis,the metabolic fingerprint was established.Multivariate statistical analysis was used to analyze the differences of metabonome among the control,model and Baixiangdan Capsule treated groups.RESULTS The significant changes of endogenous metabolites were found in the three groups and the potential biomarkers,including glucocorticoids,estrogens,neurotransmitters,and amino acids were identified.The contents of epinephrine,tetrahydrodeoxycorticosterone,5a-tetrahydrocortisol,estrone,lysine,5-hydroxylysine,acetylcysteine in rat serum increased in model group,and adjusted in the Baixiangdan Capsule group.The multi-target effect was discovered in the Baixiangdan Capsule group.CONCLUSION The metabonomics method may be applied to the study of metabolic pattern in premenstrual syndrome with liver-qi invasion and the treatment effect of TCM and it is helpful for clinical diagnosis and therapy.