复元胶囊对大鼠膝骨关节炎软骨Ⅰ、Ⅱ型胶原及蛋白多糖的影响

Effect of Fuyuan Capsule on collagen typeⅠ、collagen typeⅡ and proteoglycans in articular cartilage of rat knee osteoarthritis

目的观察复元胶囊对大鼠膝骨关节炎(OA)软骨Ⅰ、Ⅱ型胶原及蛋白多糖的影响,探讨其保护关节软骨的作用机理。方法采用Hulth法建立OA模型,随机分为6组:正常组、模型组、四环素组及复元胶囊低、中、高剂量组。各药物组分别给不同剂量药物灌胃每天1次,持续12周。免疫组化检测软骨Ⅰ、Ⅱ型胶原表达,甲苯胺蓝染色软骨蛋白多糖含量。结果复元胶囊各组软骨Ⅱ型胶原及蛋白多糖显著高于模型组(P<0.01),而Ⅰ型胶原低于模型组(P<0.05)。结论复元胶囊能增加大鼠膝骨关节炎软骨中Ⅱ型胶原表达及蛋白多糖水平,同时降低Ⅰ型胶原表达,对维持正常胶原表型、保护关节软骨有一定的作用。

AIM To investigate the effect of Fuyuan Capsule on the expression of collagen type Ⅰ、 collagen type Ⅱ and proteoglycans in articular cartilage of rat knee osteoarthritis（OA） as well as to further explore the mechanism how it prevents in protection of articular cartilage against OA.METHODS OA model was induced by Hulth＇s method and rats were randomly divided into 6 groups： normal group,model group,tetracycline group,Fuyuan Capsule low-dose group,Fuyuan Capsule middle-dose group and Fuyuan Capsule high-dose group.Subsequently,the treated groups were given different dose of drugs administered once a day for 12 weeks.Moreover,the expression of collagen type Ⅰ and collagen type Ⅱ in cartilage was detected by immunohistochemistry and the level of proteoglycan in cartilage was tested by staining with toluidine blue.RESULTS Compared with the model group,the Fuyuan Capsule group showed higher expression level of collagen type Ⅱ and proteoglycans（P0.01）,simultaneously,had lower expression level of collagen type Ⅰ（P0.05）.CONCLUSION Fuyuan Capsule can enchance the expression of collagen type Ⅱ and proteoglycans,reduce the expression of collagen type Ⅰ,which would maintain the normal collagen phenotype and protect articular cartilage.