枳实白芍提取物配伍主成分的肠吸收变化研究

Intestinal absorption of main components in the extracts from Aurantii immaturus Fructus and Paeoniae Radix

目的以枳实和白芍中主要活性成分柚皮苷和芍药苷为指标,考察枳实提取物和白芍提取物配伍后这两种成分在大鼠肠道中的吸收情况。方法采用大鼠在体肠灌流模型,应用超高效液相色谱(UPLC)测定枳实白芍提取物配伍后柚皮苷和芍药苷在肠道吸收的变化量。结果枳实提取物中柚皮苷在十二指肠、空肠、回肠和结肠的吸收率分别为6.18%、5.47%、4.64%、5.12%,白芍提取物中芍药苷在上述四个肠段的吸收率分别为8.08%、7.42%、7.13%、6.66%。结论枳实白芍配伍后柚皮苷和芍药苷与单独提取的提取物相比在大鼠肠道的吸收并未发生显著变化。

AIM To investigate the rat intestinal absorption and interaction of two bioactive components,naringin and paeoniflorin in the extracts of Aurantii immaturus Fructus and Paeoniae Radix.METHODS The rat enteroclysis model was employed and the concentration of naringin and paeoniflorin in intestinal samples were determined by Ultra Performance LC.RESULTS The percent absorption of naringin in its extract was 6.18%,5.47%,4.64% and 5.12% in duodenum,jejunum,ileum and colon in rats,whereas the percent absorption of paeoniflorin in its extract was 8.08%,7.42%,7.13% and 6.66% in rats,respectively.CONCLUSION The absorption of naringin and paeoniflorin in intestine has no significant difference between single extract and the mixture of two extracts.