窈窕茶的质量标准研究

Quality standard for Manyokin Tea

目的建立窈窕茶(决明子,枳实,厚朴,陈皮等)的质量标准。方法采用薄层色谱法鉴别窈窕茶中决明子、枳实及厚朴;采用反相高效液相色谱法测定窈窕茶中厚朴酚及和厚朴酚。采用DiamonsidTM-C18色谱柱,甲醇-0.3%磷酸(68∶32)为流动相、体积流量1.0 mL/min、检测波长294 nm、柱温30℃。结果薄层斑点清晰;厚朴酚线性范围44.4～111.0μg/mL(r=0.999 3)、和厚朴酚线性范围15.0～45.0μg/mL(r=0.999 1);厚朴酚及和厚朴酚方法精密度RSD分别为1.28%及1.52%(n=6);12 h内稳定性RSD分别为0.95%及1.24%;重复性中厚朴酚平均质量分数为0.88 mg/g,RSD为1.83%;和厚朴酚平均质量分数为0.33 mg/g,RSD为2.30%;平均回收率分别为99.2%和96.2%。结论所建立的方法准确、可靠、专属性强,说明本法可用于窈窕茶的质量控制。

AIM To establish the quality standards for Manyokin Tea（Cassiae Semen,Aurantii Fructus immaturus,Magnoliae officinalis Cortex,Citri Reticulatae Pericarpium,etc.）.METHODS Cassiae Semen,Aurantii Fructus immaturus and Magnoliae officinalis Cortex were identified by TLC.A RP-HPLC method was established to determine magnolol and honokiol in Manyokin Tea.The analytical column was DiamonsidTM-C18 column.The mobile phase was methanol-0.3% phosphoric acid（68∶ 32） with the flow rate of 1.0 mL/min.The detection wavelength was set at 294 nm.The column temperature was 30 ℃.RESULTS The TLC spots developed was clear.The linear range of magnolol was 44.4-111.0 μg/mL（r=0.999 3） and that of honokiol was 15.0-45.0 μg/mL（r=0.999 1）.The RSDs of the method precision were 1.28% and 1.52%（n=6）.The RSDs of stability in 12 h were 0.95% and 1.24%.The average content of magnolol was 0.88 mg/g（RSD was 1.83%）,and honokiol was 0.33 mg/g（RSD was 2.30%） in repeatability test.The average recoveries of magnolol and honokiol were 99.2% and 96.2%,respectively.CONCLUSION This method is accurate and reliable,can be used for quality control of this preparation.