大薯微型块茎的离体诱导

Induction of the Microtuber of Dioscorea alata L. in Vitro

研究IBA、BA、蔗糖、光照以及昼夜温差对大薯(Dioscorea alata L.)微型块茎诱导形成的影响,确定影响大薯微型块茎形成的重要因素,筛选最佳培养方案。以大薯组织培养不定芽为材料,研究不同植物激素浓度、蔗糖含量、光照时间和昼夜温差对大薯微型块茎形成时间、形成率、平均微块茎数和直径的影响。添加植物激素能促进大薯微型块茎提早成形,IBA和BA对大薯微型块茎的形成表现为增效作用;蔗糖浓度对大薯微型块茎的成形具有显著作用,在不添加蔗糖的培养基中,没有成形的大薯微型块茎,适当增加蔗糖浓度能提高大薯微型块茎的形成率,而高浓度的蔗糖则会抑制大薯微型块茎的形成和生长。以IBA2.0mg/L+BA0.5mg/L,蔗糖浓度为60g/L时,所诱导的微型块茎较多且大,为诱导大薯微型块茎的最佳培养基组合。短光照和昼夜温差处理也能显著提高微型块茎的诱导率,数量和大小。植物激素、蔗糖浓度、光照和昼夜温差均为影响大薯微型块茎形成的重要因素。IBA和BA配合作用时表现互作效应,而其互作作用也受蔗糖浓度和光温条件的影响。是否添加蔗糖直接决定有无大薯微型块茎的形成。

By studying the effect of IBA,BA,sucrose,short-illumination and difference temperature of day and night on the induction of microtuber of Dioscorea alata L.,to determine the impact factors of the microtuber ＇ s farmation and screening the best training schemes.With Dioscorea alata L.tissue culture in vitro shoot as materials,research the influence of plant hormones,sucrose concentration,the time of light and between day and night processing on Dioscorea alata L.microtuber＇s formation.Add plant hormones can promote the Dioscorea alata microtubers formed early,IBA and BA play a role of synergy.It was proved that appropriate sucrose concentration had significant effect on the farmation of Dioscorea alata L.microtubers,no or low level of sucrose causes none forming microtubers and high levels of sucrose lead to inhibition on the growth of Dioscorea alata L..The suitable concentration that induced more and bigger microtubers was IBA 2.0 mg/L ＋ BA 0.5 mg/L ＋ sucrose 60 g/L.Short light and day and night temperature could also improve the microtuber induction rate,numbers and sizes.Plant hormones,day and light temperature,sucrose concentration were important factors for microtubers formation.The interaction was existed between IBA and BA,and it was affected by sucrose concentration,illumination and temperature conditions.Sucrose existion decided microtubers formation directly.