大黄蛰虫丸对肝纤维化大鼠肝α-SMA表达的动态观察

Dynamic changes of modified Dahuangzhechongwan on the expression of α-SMA in hepatic fibrosis rats

目的观察大黄蜇虫丸对免疫性肝纤维化大鼠肝脏α-SMA表达的动态变化,探讨大黄蜇虫丸抗肝纤维化的作用机制。方法将120只健康雄性SD大鼠随机分为6组,即正常对照组,模型组,大黄蜇虫丸低、中、高剂量组,秋水仙碱组各20只。除正常对照组外其他各组均采用注射人血清白蛋白制作免疫性肝纤维化大鼠模型,模型制作完成后,各组采用相应的药物干预,连续4周,分别于药物治疗后1,2,3,4周末随机处死5只大鼠,用免疫组化技术检测肝脏组织中α-平滑肌肌动蛋白(α-SMA)的表达变化。结果①模型组随着周次的延长肝脏组织中α-SMA表达及胶原纤维含量同步增高,有统计学意义(P<0.01);②大黄蜇虫丸低、中、高剂量组随着周次的延长肝脏组织中α-SMA表达及胶原纤维含量同步降低,有统计学意义(P<0.01),但大黄蜇虫丸中剂量组优于其他2组(P<0.01);③大黄蜇虫丸有明显的抑制肝纤维化模型大鼠肝组织α-SMA的表达作用,且抑制作用要优于秋水仙碱(P<0.01)。结论大黄蜇虫丸可有效防治大鼠免疫性肝纤维化,其作用机制可能是通过抑制肝纤维化组织α-SMA的表达来实现的。

Objective To observe the dynamic changes of modified Dahuang zhechong pill（DAZCP） on α-smooth muscle actin in thepathological immune hepatic fibrosis rats and to research the mechanism of Dahuangzhechong pill on anti-hepatic fibrosis.Methods 120 health male SD rats were randomly divided into six groups： control group,model group,DAZCP low-dose group,medium-dose group,high-dose group and colchicines group.Except for control group,model rats were made by injecting human serum albumin.All rats had been administered for 4 weeks.After 1,2,3,4 weeks,detected the expression of α-SMA by immunohistochemistry and observe the hepatic structure.Results ① The results showed that the α-SMA and collagen increased significantly in model group with time extend（P0.01）.②The α-SMA and collagen decreased significantly in DAZCP low-dose group,medium-dose group and high-dose group with time extend（P0.01）.The medium-dose group was better than the other groups（P0.01）.③Pill of Dahuangzhechong inhibited obviously the expression liver of α-SMA in hepatic tissue of rat with hepatic fibrosisin comparison than in colchicines.Conclusion Pill of Dahuangzhechong can prevent and treat hepatic fibrosis rat and the mechanism maybe inhibit the expression liver of α-SMA in hepatic tissue.