草莓未受精子房的离体培养

Culture in vitro of Unfertilized Ovary in Strawberry (Fragaria spp.)

花药培养和未受精子房或胚珠离体培养是人工诱导形成单倍体植株的2条途径。草莓染色体倍性复杂多样,为创新育种资源,本研究以草莓为试材,分析未受精子房的离体培养过程中基因型、外源激素、蔗糖浓度、低温预处理、生长环境及发育状况、光照条件6个关键因素对诱导雌核发育的影响。结果表明:选择合适基因型的露地栽培的一级花蕾,外源激素添加2,4-D,蔗糖浓度6%,低温预处理48h,黑暗变温处理是草莓未受精子房离体培养获得单倍体胚囊植株的理想条件。本研究为草莓倍性育种提供参考。

Anther culture and culture in vitro of unfertilized ovary or ovule are two methods to artificially induce haploid plantlets strawberry are with complicated ploidy.For the purpose of creating novel breeding materials,we employed strawberry as experimental materials,analyzed effects of six key factors,including genotype,extrinsic hormone,sucrose concentration,cold pretreatment,growth environment and development status,and illumination condition on induction of gynogenesis in vitro of unfertilized ovary.Results showed that the primary bud of land plants with appropriate gene type,2,4-D as extrinsic hormone,the concentration of sucrose at six percentage and cold pretreatment for 48 hours,were the optimum conditions for haploid plants by embryo sac induction of unfertilized ovary in strawberry.The research aimed at providing reference for ploidy breeding in strawberry.