IFN-γ调节THP-1细胞应答沙门氏伤寒菌

Regulation of THP 1 cells by IFN γ in response to Salmonella typhi strains

目的：为了探讨ＩＦＮγ在增强宿主防御沙门氏伤寒菌感染中的作用机制。方法：采用体外细胞培养方法研究了ＩＦＮγ对ＴＨＰ１细胞应答沙门氏伤寒菌的调节作用。结果：发现ＩＦＮγ显著促进ＴＨＰ１细胞及ＰＭＡ分化的ＴＨＰ１细胞内化和杀伤沙门氏伤寒菌，并明显增强沙门氏伤寒菌诱导ＰＭＡ分化的ＴＨＰ１细胞产生ＴＮＦα和增强诱导ＴＨＰ１细胞及ＰＭＡ分化的ＴＨＰ１细胞产生ＩＬ１２。

Objective:To research the enhancing mechanisms of host defence against Salmonella typhi infection by IFN γ.Methods:The regulatory role of THP 1 cells by IFN γ in response to Salmonella typhi was researched in vitro.Results:IFN γ significantly enhanced the activities of THP 1 cells and PMA differentiated THP 1 cells to internalize and kill Salmonella typhi strains, and also increased the production of TNF α induction in PMA differentiated THP 1 cells and the production of IL 12 induction by Salmonella typhi strains in THP 1 cells and PMA differentiated THP 1 cells.Conclusion:IFN γ plays an important role in the regulation of mononuclear phagocytes in response to Salmonella typhi infection.