环磷酰胺诱发大鼠胸腺细胞凋亡的研究

Induction of apoptosis in rat thymus by cyclophosphamide

目的：研究环磷酰胺的免疫抑制作用机制。方法：以大鼠胸腺为对象，采用细胞凋亡的原位特异性染色及ＤＮＡ凝胶电泳的方法，观察不同剂量、不同时间环磷酰胺处理后胸腺细胞的凋亡现象。结果：剂量为２０和７０ｍｇ／ｋｇ的环磷酰胺可引起广泛的胸腺细胞凋亡。该作用最早发生在环磷酰胺处理后的８ｈ，１２～２４ｈ最明显，４８ｈ消失。结论：环磷酰胺的免疫抑制作用可能是通过诱导免疫细胞凋亡而产生的。

Objective:To understand the mechanism of immunosuppression of cyclophosphamide. Methods: Investigate if cyclophosphamide produces the immunosuppression by the induction of apoptotic cell death in thymus .Results: 20 and 70 mg/kg cyclophosphamide could induce markedly apoptosis in rat thymus at 12 h after treatment ,the apoptotic cell death took place at 8～24 h and disappeared at 48 h after cyclophosphamide treatment. Conclusion: Cyclophosphamide may causes immunosuppression through the induction of apoposis of thymocyte.