血管内皮生长因子的免疫学测定法

Radioimmunoassay of vascular endothelial growth factor

目的：检测肿瘤细胞条件培养液中ＶＥＧＦ的表达量。方法：在表达及分离纯化ＶＥＧＦ的基础上，成功地制备了ＶＥＧＦ抗体，以夹心法测定ＶＥＧＦ。结果：ＶＥＧＦ测定范围为００２～２００００ｎｇ／ｍｌ，灵敏度可达００２ｎｇ／ｍｌ，批内及批间变异均小于１０％，回收率平均为１０２４％。其中人胃癌细胞ＭＧＣ８０３、ＢＧＣ８２３、乳癌ＭＣＦ７及肝癌ＢＥＬ７４０２分泌上清中ＶＥＧＦ的含量分别为６５０、０４５、０９０及１４５ｎｇ／ｍｌ。结论：不同的肿瘤细胞系其ＶＥＧＦ的分泌量是不同的，应用此方法可以检测肿瘤病人血清中的ＶＥＧＦ。

Objective: To detect expression level of VEGF is important for determining biological behaviour of tumor cells. Methods: Based on isolation, purification and expression of VEGF,successfully prepared anti VEGF antibody and developed a sandwich radioimmunoassay for VEGF. Results: Detectable range of VEGF is between 0.02 and 200.00 ng/ml.The sensitivty or minimal detectable concerntration dose was difference of sample in the same group and among groups were all less than 10 percent. By the assay it is indicated that all four kinds of tumor cell lines secreted VEGF, MGC803, BGC823, MCF 7 and BEL 7402 separeately secreted 6.50,0.45, 0.90 ,1.45 ng/ml. Conclusion: VEGF can be detected in the serum of tumor patient with this assay.