微囊化猪胰岛对特异性抗体的免疫隔离作用的研究

Study on the immunoisolation of microencapsulated porcine islet like cell clusters from special antibodies

目的：为了探讨一步成囊法制备的海藻酸钠微囊对猪胰岛样细胞团（Ｉｓｌｅｔｌｉｋｅｃｅｌｃｌｕｓｔｅｒ，ＩＣＣ）的保护作用。方法：本研究把经猪ＩＣＣ免疫的小鼠新鲜血清，与微囊化ＩＣＣ或游离ＩＣＣ共同孵育后，观察各组ＩＣＣ的胰岛素释放试验。结果：微囊ＩＣＣ组的基础及刺激后的胰岛素值分别为９３４９±１６２１，２７５００±３５１９ｍＵ／Ｌ，与对照组比较差异无显著性（Ｐ＞００５）；游离ＩＣＣ组的基础及刺激后胰岛素值分别为６６８３±１０２４，９２４１±２５３２ｍＵ／Ｌ，明显低于微囊ＩＣＣ组及对照组（Ｐ＜００１）。结论：表明免疫血清对游离ＩＣＣ有明显损害，而海藻酸钠微囊对特异性抗体有免疫隔离作用。

Objective:In order to study the protective effect of alginate microcapsules by one step encapsulated method to porcine islet like cell clusters (ICC). Methods:Microencapsulated ICC (McICC) and free ICC (FICC) were incubated with fresh sera of immunized mice by porcine ICC, then the changes of insulin release of McICC and FICC were observed. Results: The results indicated that the insulin concentrations of basal and stimulated McICC group were 93 49±16 21 , 275 00±35 19 mU/L respectively, there was no difference between McICC and control ICC ( P >0.05), and that the concentrations of basal and stimulated insulin of FICC were 66 83±10 24 , 92 41±25 32 mU/L respectively, which were significantly lower than those of McICC and control ICC ( P <0.01). Conclusion:It was suggested that immunized sera could injure FICC seriously and that alginate microcapsules had the efficient effect of immunoisolation, which could prevent ICC from the destruction of special antibodies.