哮喘病人B细胞体外产生IgE过程中幼稚性T细胞的影响作用

The effects of naive T lymphocytes on IgE prodution by B lymphocytes in patients with bronchial asthma

目的：探讨支气管哮喘发病机制中Ｔ细胞的调节作用。方法：分离出病人辅助性Ｔ细胞中的幼稚性（Ｎａｉｖｅ）Ｔ细胞亚群即ＣＤ４＋ＣＤ４５ＲＡ＋Ｔ细胞亚群，并将其与各自的Ｂ淋巴细胞共同培养。同时设定非特异性刺激原ＰＷＭ（美洲商陆）刺激组及非刺激组，测定培养上清液中ＩｇＥ含量。结果：非刺激组病人ＩｇＥ含量明显高于健康对照（Ｐ＜００１），刺激组病人与健康对照ＩｇＥ含量无显著差别（Ｐ＞００５）。

Objective:To study the role of naive T lymphocytes in the development of asthma. Method: The authors isolated naive T lymphocytes (CD4 +CD45RA + T cell) from peripheral blood of patients with bronchial asthma and health donors and culture them with autodogous B cells. The level of IgE in the supernatant from cultured cells conditioned with PWM was determined. Results:Secreted IgE in the supernatant from patients was significantly higher than that from normal donors in non stimulated group. There are not difference in IgE production between patients and normal donors in PWM stimulated group. Conclusion:It suggests that naive T lymphocytes from patients with bronchial asthma have a positive effect on secreting IgE.