以HBV· DNA作为指标对HBV感染状态的调查研究

Investigation of the HBV·DNA as a Valuable Marker of HBV Infection

目的 以 Ｈ Ｂ Ｖ· Ｄ Ｎ Ａ 作为 Ｈ Ｂ Ｖ感染的流行病学调查指标。方法 应用 Ｐ Ｃ Ｒ技术对１０ ９７６ 例肝病患者血清中的 Ｈ Ｂ Ｖ· Ｄ Ｎ Ａ 进行检测，并与其它乙肝病毒标志物（ Ｈ Ｂ Ｖ Ｍ）尤其是 Ｈ Ｂｅ Ａｇ 进行对比分析。结果 Ｈ Ｂ Ｖ· Ｄ Ｎ Ａ 阳性率（４１．８％ ）明显高于 Ｈ Ｂｅ Ａｇ 阳性率（２２．５％ ）。从 Ｈ Ｂ Ｖ· Ｄ Ｎ Ａ 检出率的性别与年龄分布来看，男性组（４５．４％ ）明显高于女性组（２９８％ ），６０ 岁以上年龄组和２０ 岁以下年龄组阳性率较低（３３５％ 、３７４％ ），其它几个年龄组较高且较接近（４１７％ 、４３０％ 、４４０％ 、４２４％ ）（ Ｐ＞ ０．０５）。不同类型肝病患者血清 Ｈ Ｂ Ｖ· Ｄ Ｎ Ａ 的检测为：肝硬化组 Ｈ Ｂ Ｖ· Ｄ Ｎ Ａ 阳性率最高（６４．５％ ），慢性肝炎组次之（５７．１％ ），急性肝炎组最低（２５．６％ ）。结论 以 Ｐ Ｃ Ｒ技术检测 Ｈ Ｂ Ｖ· Ｄ Ｎ Ａ 明显较其它乙肝病毒标志物敏感，它可进一步揭示 Ｈ Ｂ Ｖ 的传染源和传播途径，也更有利于临床对乙肝的诊断和治疗。

Objective The HBV·DNA was used to be the epidemiological investigation markers of HBV infection. Methods The PCR was used to detect HBV·DNA which was compared with other HBV markers in 10 976 serum samples of patients, especially HBeAg. Results The positive rate of HBV·DNA was 41.8% which was obviously higher than that of HBeAg (22.5%). According to the positive rate of HBV·DNA based on the age and sex, more cases were found in the male (45.4%) than in the female (29.8%). Less cases were found in the group over 60 years old (33.5%) and under 20 years old (37.4%). Other groups were higher and more colse (41.7%,43.0%.44.0%,42.4%) (P>0 05). The positive rate of HBV·DNA of the group with liver cirrhosis was the highest(61.5%),whcih of the group with chronic hepatitis was the second (57.1%) and the lowest is the group with acute hepatitis (25.6%). Conclusion The test of HBV·DNA by PCR was more sensitive than that of other HBV markers, which can reveal the transmitting resourses and path of HBV and can be useful in the diagnosis and treatment of hepatitis B clinically. [