引进国外蓝莓品种的组织培养及快繁技术研究

Study on the Techniques of Tissue Culture and Rapid Propagation of Introduced Foreign Blueberry Varieties

以从保加利亚引进的蓝莓品种B91和B92的未萌动枝条为试验材料,进行了顶芽、茎尖和茎段的萌发与分化、茎段的继代丛生与快繁、试管苗生根与移栽等技术的研究。结果表明:对未萌动枝条进行预处理能明显提高接种效率;WPM+ZEA 2.0 mg.L-1是接种茎段的适宜培养基,WPM+ZEA 1.0 mg.L-1是大量产生丛生芽的快繁培养基,1/2 WPM+IBA 2.0 mg.L-1+NAA 0.5 mg.L-1是生根的理想培养基;在温室中草炭、河砂和园土按1∶1∶1混合,经过硫粉调酸后是试管苗移栽的较好基质;移植到示范果园的试管苗长势旺盛。

Dormant shoot of introduced blueberry varieties of B91 and B92 from Bulgaria were used as experimental materials to carry out a study on techniques of budding and dissimilating of apical bud,stem apex and stem segments,regeneration and fast propagation of stem segments,rooting and transplanting of seedlings in test tube.The results showed that the pre-treatment on dormant shoot has significantly increased efficiency of inoculation;WPM＋ZEA 2.0 mg·L-1 was a suitable medium for stem segment inoculation;WPM＋ZEA 1.0 mg·L-1 was a fast propagation medium producing a large number of clusters;1/2 WPM＋IBA 1.0 mg·L-1＋NAA 0.5 mg·L-1 was the ideal medium for rooting.In the greenhouse peat,river sand and garden soil mixed at 1∶1∶1 ratio,and treated with sulphur powder after acid was a comparatively good substrate for transplantation of seedlings produced in test tube;Transplanted seedlings performed vigorous growth in the demonstrate orchard.