不同剂量的谷氨酰胺对大鼠应激性溃疡的保护作用及对皮质醇 β-内啡肽的影响

Protection of different doses of glutamine on stress ulcer and their effect on serum cortisol, β-endorphin in rats

目的 探讨不同剂量的谷氨酰胺（glutamine,Gln）对大鼠应激性溃疡的保护作用及对血清皮质醇、β-内啡肽（β-endorpin,β-EP）的影响.方法 64只SD大鼠随机分为对照组（N组）、应激组（S组）和Gln G1～G6组,每组8只,应用束缚浸水应激（WRS）方法制作大鼠应激性溃疡模型,检测各组大鼠胃液pH值、溃疡指数（UI）值、H＋-K＋-ATP酶活性,抽取各组血清测定皮质醇、β-EP水平.结果 G2～G6组胃液pH值、H＋-K＋-ATP酶活性与S组比较差异有统计学意义（P〈0.05）,G1～G6组UI值与S组比较差异有统计学意义（P〈0.05）,G4～G6组间胃液pH值、UI值、H＋-K＋-ATP酶活性比较差异均无统计学意义（P〉0.05）.G5、G6组皮质醇与S组比较差异有统计学意义（P〈0.05）,G6组β-EP与S组比较差异有统计学意义（P〈0.05）.结论 补充适量的Gln能减少应激大鼠的胃酸分泌,降低血清皮质醇、β-EP水平,减轻应激性溃疡程度.

Objective To study the protective effect of different doses of glutamine on stress ulcer, and their effect on serum cortisol,β - endorphin in rats. Methods 64 SD rats were randomly divided into control group （ N group, 8） , stress group （ S group, n = 8 ） and glutamine 1 to 6 groups （G1 - G6 groups, n = 8）. Stress ulcer was induced in rats by water immersion restraint stress（ WRS）, the gastric juice pH, the size of ulcer index （ UI ） and H ＋ - K ＋ - ATP activity were measured, the concentrations of serum cortisol and β - endorphin were determined. Results The gastric juice pH value, H＋ -K＋ -ATP activity were significantly different between the G2 -G6 group and the S group （P 〈 0.05 ）, the UI value of G1 - G6 group were significantly lower than those of the S group （ P 〈 0. 05 ） ;There were no significant differences in the gastric juice pH value, ulcer index size （UI）and the H＋ - K ＋ - ATP activity of the G4 - G6 group （ P 〉 0.05 ）. The serum cortisol value of the GS, G6 group were significantly lower than those of the S group （ P 〈 0.05 ） ; the serum β - endorphin value of the G6 group were significantly loser than those of the S group （ P 〈 0.05 ）. Conclusion The amount of glutamine supplement can reduce the stress gastric acid secretion, decrease serum cortisol anti β- endorphin levels, and reduce the degree of stress ulcer.