RP-HPLC双波长切换法同时测定复方酸枣仁颗粒中芒果苷和斯皮诺素

RP-HPLC Simultaneous Determination of Mangiferin and Spinosin in Ziziphus Jujuba Mill Granules under Double UV Wavelengths

目的:建立复方酸枣仁颗粒中芒果苷和斯皮诺素含量测定的反相高效液相色谱方法。方法:采用液-液萃取法纯化样品。采用Hypersil C18色谱柱(4.6 mm×250 mm,5μm),以乙腈-1%冰醋酸水系统作为流动相,梯度洗脱;波长检测程序0～25 min,258 nm,25～35 min,335 nm;流速1.0 mL·min-1;柱温30℃。结果:在上述色谱条件下,测得芒果苷和斯皮诺素分别在4.320×10-3～5.200×10-2g.L-1(r=0.999 4)和5.200×10-3～1.040×10-1g.L-1(r=0.999 7)线性关系良好;芒果苷和斯皮诺素的平均回收率(n=9)分别为94.6%(RSD 1.9%)和95.1%(RSD 2.3%)。结论:方法操作简便,准确,重复性良好,可用于复方酸枣仁颗粒的质量控制。

Objective：To develop a RP-HPLC method for determination of mangiferin and spinosin in Ziziphus Jujuba Mill granules.Method：Samples were purified by Liquid-Liquid extraction.Hypersil C18（4.6 mm×250 mm,5 μm） column was used.The mobile phase consisted of acetonitrile and water with 1% glacial acetic acid;the flow rate was 1.0 mL·min-1.Detection wavelengths were set at 258 nm,and switched to 335 nm at 25 min.Result：The linear response ranges were 4.320×10-3-5.200×102g·L-1（r=0.999 4） and 5.200×10-3-1.040×10-1g·L-1（r=0.999 7）for mangiferin and spinosin respectively.The average recoveries（n=9）of these compounds wre 94.6%（RSD 1.9%） and 95.1%（RSD 2.3%）respectively.Conclusion：A simple,accurate and reproducible method is provided for simultaneous determination of mangiferin and spinosin in Ziziphus Jujuba Mill granules by RP-HPLC.