HPLC-ELSD测定黄芪提取物中黄芪皂苷Ⅰ,Ⅱ及乙酰黄芪皂苷

Determination of Astragaloside Ⅰ,Ⅱ and Acetytastragaloside Ⅰ in Astragalus Extract by HPLC-ELSD

目的:研究以HPLC-ELSD法测定黄芪提取物中黄芪皂苷Ⅰ,Ⅱ,乙酰黄芪皂苷Ⅰ的含量。方法:色谱柱为WelcC18(4.6 mm×250 mm5,μm),流动相为乙腈和水,梯度洗脱,流速1 mL·min-1,柱温30℃,漂移管温度80℃,气体(空气)压力为50 psi。结果:黄芪皂苷Ⅰ,Ⅱ,乙酰黄芪皂苷Ⅰ的平均回收率分别为101.16%,99.34%,100.43%,RSD分别为2.76%,2.28%,2.55%,含量分别为4.01%,1.70%,3.67%。结论:方法简单易行,结果可靠,分离效果好,可作为黄芪提取物中皂苷类成分定量的方法。

Objective：To establish an HPLC-ELSD method to determine the content of astragaloside I,II and acetylastragaloside I in Astragalus extract.Method：The HPLC-ELSD system consisted of Welc C18 column（4.6 mm×250 mm,5 μm） with the mobile phase consisting of acetonitrile-water using a gradient elution.The flow rate was 1 mL·min-1 and the column temperature was at 30 ℃.The drift tube temperature of ELSD was 80 ℃ with a carrier gas（air） pressure of 50 psi.Result：The average recoveries of astragaloside I,II and acetylastragaloside I were 101.16%,99.34% and 100.43% respectively,with RSD of 2.76%,2.28%,2.55%.The content of astragaloside I,II and acetylastragaloside I was 4.01%,1.70%,3.67% respectively.Conclusion：It was proved that the newly-established HPLC-ELSD method was simple,reliable,well-separated and precise,which could be used for quantitative analysis of saponins in Astragalus extract.