菝葜中的甾体皂苷及其细胞毒活性

Studies on Steroidal Saponins from Smilax china and Their Cytotoxic Activities

目的:研究菝葜Smilax china L.的甾体皂苷类成分及其细胞毒活性。方法:利用色谱技术进行分离纯化,并根据化合物的理化性质和光谱数据进行结构鉴定。采用MTT法对分离得到的单体化合物进行细胞毒活性的初筛。结果:从该植物乙醇提取物的正丁醇部分分离得到6个甾体皂苷,分别鉴定为borassoside B(1),薯蓣皂苷(2),原薯蓣皂苷(3),22-O-甲基原薯蓣皂苷(4),isonarthogenin 3-O-α-L-rhamnopyranosyl(1-2)-O-[α-L-rhamnopyranosyl-(1-4)]-β-D-glucopyranoside(5),diosgenin-3-O-[α-L-rha(1-3)-α-L-rha(1-4)-α-L-rha(1-4)]-β-D-glucopyranoside(6)。在样品溶液质量浓度为5 mg.L-1时,化合物2和6对K-562癌细胞生长的抑制率分别是89.1%9,0.1%。结论:化合物1和6是首次从该植物中分离得到,也是首次从菝葜属植物中分离得到。化合物2和6对K-562癌细胞具有明显的体外细胞毒活性。

Objective：To study the chemical constitutes of Smilax china and the cytotoxic activities of isolated compounds.Method：Various chromatographic techniques were used to separate and purify the constituents.Their structures were established on the basis of their physico-chemical properties and spectral data.The isolated compounds were evaluated for their cytotoxic activities by MTT method.Result：Six steroidal saponins were isolated from the dried rhizomes of S.china.Their structures were established on the basis of spectroscopic evidence（NMR,MS） as borassoside B（1）,dioscin（2）,protodioscin（3）,22-O-methyl protodioscin（4）,isonarthogenin 3-O-α-L-rhamnopyranosyl（1-2）-O-[α-L-rhamnopyranosyl-（1-4）]-β-D-glucopyranoside（5）,diosgenin-3-O-[α-L-rha（1-3）-α-L-rha（1-4）-α-L-rha（1-4）]-β-D-glucopyranoside（6）.Compounds 2 and 6 showed significant cytotoxic activities against K-562 carcinoma cell line at the concentration of 5 mg·L-1.The inhibitory percentage of compounds 2 and 6 on K-562 cell growth were 89.1% and 90.1% at concentration of 5 mg·L-1.Conclusion：Compounds 1 and 6 were isolated from this plant and Smilax for the first time.Compounds 2 and 6 showed cytotoxic activities against K-562 carcinoma cell line in vitro.