高血压病肝阳上亢证尿液代谢组学气相色谱-质谱联用研究

Study of Hypertension Patients with Syndrome of Ascendant Hyperactivity of Liver Yang on Urine Metabonomics

目的:分析尿液代谢物变化,寻找高血压病肝阳上亢证的特征代谢物,揭示高血压病肝阳上亢的证候本质。方法:根据临床高血压病肝阳上亢证的诊断标准,选择典型病例9例,健康志愿者11例,取其尿液经衍生化,以GC-MS采集高血压病肝阳上亢证患者和健康志愿者的尿液代谢物谱,数据经匹配、对齐、共有特征选择,利用Simca-P软件进行主成分分析(PCA,principal component analysis)和偏最小二乘判别分析(PLS-DA,partial least squares discriminant analysis),寻找可能的生物代谢物,并做出相关代谢途径的可能解释。结果:PCA及PLS-DA方法处理高血压病肝阳上亢证患者和健康志愿者的尿液代谢物谱数据显示,两组GC-MS谱数据可以明显分开,与健康志愿者作比较,患者机体相关代谢发生显著变化,体内硬脂酸、肌醇、D-果糖、邻苯二甲酸代谢明显异于健康志愿者。结论:GC-MS结合模式识别的代谢组学方法具有研究复杂条件下机体病理生理变化的优势,为理解高血压病肝阳上亢证的证候本质和诊断该类病证提供了科学依据。

Objective：Analysis the difference of urine metabolites between hypertension patients with the syndrome of ascendant hyperactivity of liver yang and healthy volunteers,search the possible biomarker,reveal the nature of the syndrome of ascendant hyperactivity of liver yang.Method：According to the clinical diagnostic criteria of the syndrome of ascendant hyperactivity of liver yang,select typical cases,select nine typical cases and eleven healthy volunteers,analysis the components in the urine derivatives of hypertension patients with the syndrome of ascendant hyperactivity of liver yang and healthy volunteers by gas chromatography mass spectrometry,match and align the data,select the same features,make the PCA and PLS-DA by the software Simca-P,track biomarkers and explain the possible relevant metabolic pathways.Result：The data from the analysis by PCA and PLS-DA of the urine samples shows that：two sets of data from GC-MS can be classified in the score plot.Some significantly changed metabolites like stearic acid,inositol,D-fructose,phthalic acid have been identified between the two groups in terms of relative metabolism of body.Conclusion：The metabonomics method,gas chromatography-mass spectrometry combined with pattern recognition,has advantages in studying pathophysiological changes of the body under the complicated conditions.The study provided a scientific basis for the the essence of traditional chinese medicine syndrome theory and clinical diagnosis of the syndrome of ascendant hyperactivity of liver yang.