大苞雪莲醇提物的抗缺氧作用及其初步机制研究

Anti-hypoxia Effects of Ethanol Extract from Saussurea involucrate

目的:研究大苞雪莲醇提物的抗缺氧作用及其机制。方法:将BALB/c小鼠随机分为缺氧模型组大苞雪莲醇提物高低剂量(500,250 mg·kg-1)组及阳性药(乙酰唑胺300 mg·kg-1)对照组,单次ip给药,通过常压密闭缺氧试验、急性减压缺氧试验,化学物质中毒缺氧试验,观察ip大苞雪莲醇提物,小鼠在不同缺氧状态下的存活时间;并模拟高原低压低氧环境测定小鼠血浆中超氧化物歧化酶(SOD)、乳酸脱氢酶(LDH)活性及丙二醛(MDA)、乳酸(LD)含量。结果:与缺氧模型组相比,大苞雪莲醇提物5002,50 mg·kg-1均能显著延长不同缺氧状态下小鼠的存活时间(P<0.01),其中常压密闭缺氧模型小鼠存活时间及延长率分别为:(37.09±4.25)min,(32.36±4.22)min及44.36%,23.93%。血浆生化指标测定结果表明,大苞雪莲醇提物500,250 mg·kg-1均能提高模拟高原低压低氧环境下小鼠血浆中SOD(126.31±19.18),(120.45±13.78)U.mL-1和LDH(11 334±212.3),(10 882±173.5)U.L-1活性,降低MDA(3.21±0.52),(3.52±0.33)μmol.L-1和LD(2.37±0.44),(2.92±0.36)nmol.L-1含量(P<0.01)。结论:大苞雪莲醇提物具有明显的抗缺氧作用,其机制可能与降低血乳酸水平、促进细胞无氧酵解和改善体内氧自由基代谢的作用有关。

Objective：To investigate the effect and mechanism of anti-hypoxia components from Saussurea involucrata.Method：The BALB/c mouse models of normobaric hypoxia,decompression hypoxia and chemistry poisoning hypoxia were developed to evaluate the survival time of mice in hypoxia,and the biochemical indicators,including superoxide dismutase（SOD）,malondialdehyde（MDA）,lactic acid（LD） and lactate dehydrogenase correlated with anti-hypoxia were measured.Result：Compared to the model group,the ethanol extract of S.involucrata at the dosage of 500,250 mg·kg-1 dose-dependently enhanced the survival time of mice in different hypoxia models.At the same time,they could increase SOD（126.31±19.18）,（120.45±13.78） U·mL-1 and lactate dehydrogenase LDH activities（11 334±212.3）,（10 882±173.5） U·L-1,and decrease the content of MDA（3.21±0.52）,（3.52±0.33） μmol·L-1,and LD（2.37±0.44）,（2.92±0.36） nmol·L-1 in blood plasma of mice under a simulated decompression and hypoxia conditions.Conclusion：These results demonstrated that the ethanol extract of S.involucrata possessed notable anti-hypoxic activity.The mechanism may be related to decrease the level of LD,promote the process of anaerobic glycolysis and improve the metabolism of oxygen radicals to the body.