黄芩炭对血热出血大鼠止血有效部位研究

Haemostatic Conponent of Carbonized Scutellariae Radix on Blood of Fevered and Bleeding Rats

目的:比较黄芩生品、炭品止血效果,筛选黄芩炭有效止血部位,通过血热出血动物模型探讨该部位发挥止血作用的机制。方法:取SD大鼠60只,随机分为6组,空白对照组、模型对照组、云南白药组、黄芩生品组、黄芩炭品组、黄芩炭氯仿部位组。将供试品按每日20 mL.g-1的剂量灌胃(ig),1次/d,给药7 d。通过测定其凝血酶原时间(PT)、凝血酶时间(TT)、活化部分凝血活酶时间(APTT)、血浆纤维蛋白(FIB)4项指标及血液流变学相关指标,观察黄芩生品、炭品及各部位对模型动物血液系统的影响。结果:模型对照组较空白对照组有显著差异,显著延长PT(17.26±1.73)s,TT(23.15±1.83)s,APTT(22.43±1.93)s及高切黏度(5.72±0.55)mPa.s,中切黏度(7.44±0.78)mPa.s,低切黏度(6.59±1.10)mPa.s。黄芩炭和黄芩炭氯仿部位能显著缩短血热出血动物模型的PT,TT,APTT。黄芩炭和黄芩炭氯仿部位依次为PT(14.53±1.45)s,(13.24±1.46)s;TT(20.91±1.15)s,(18.70±1.44)s;APTT(18.07±0.75)s,(15.59±1.74)s;与模型对照组比较P<0.05或P<0.01;对FIB均没有显著影响。结论:黄芩炭氯仿部位为黄芩炭止血的有效部位,止血主要通过内源性、外源性凝血酶及内外源共同途径,与纤维蛋白系统无关。血液流变学的改善为止血效果的产生提供依据。

Objective：To compare the haemostatic mechanism of carbonized and raw Scutellariae Radix,screen effective haemostatic component from the carbonized herb by fevered and bleeding rat models.Method：SD rats were randomly divided into blank control group,model compared group,Yunnan white drug group,raw Scutellariae Radix group,carbonized Scutellariae Radix group and chloroform Scutellariae Radix group.Experimental material was used to ig dose of 20 mL·g-1 once a day,for 7 days.Prothrombin time（PT）,thrombin time（TT）,activated partial thromboplastin time（APTT）,fibrinogen content（FIB） and hemorheological parameters were examined to study the effects of experimental material on SD rats.Result：The model group had significantly difference with blank control,prolonged PT,TT,APTT,HBV,MBV and LBV to（17.26±1.73） s,（23.15±1.83） s,（22.43±1.93） s,（5.72±0.55） mPa·s,（7.44±0.78） mPa·s and（6.59±1.10） mPa·s.Carbonized Scutellariae Radix and chloroform Scutellariae Radix could significantly shorten the PT,TT and APTT of the fevered and bleeding model rats.PT,TT and APTT of carbonized Scutellariae Radix group and chloroform Scutellariae Radix group was respectively that（14.53±1.45） s,（13.24±1.46） s;（20.91±1.15） s,（18.70±1.44） s and（18.07±0.75） s,（15.59±1.74） s.Compared with the model compared group,P0.05 or P0.01.Otherwise,there was no effect on FIB.Conclusion：The chloroform extract is the effective component for hemostasis,and plays roles by ways of endogenous thrombin,extrinsic thrombin and the both,but has no relation with fibrin.The improvement of hemorheological parameters is a basis for hemostasis.