旋覆代赭汤对胃动力低下大鼠血液及组织中胃动素,胃泌素及血管活性肠肽含量的影响

Effect of Xuanfu Daizhe Decoction on Motilin,Gastrin and Vasoactive Intestinal Peptide in Blood and Tissuein Rats with Low Gastric Motility

目的:观察旋覆代赭汤对胃动力低下大鼠血液及胃窦组织中胃动素(MTL)、胃泌素(GAS)及血管活性肠肽(VIP)含量的影响。方法:将70只大鼠随机分为正常组和模型组,正常组10只,模型组60只。模型组每只大鼠按10 g·kg-1体重的甘草煎剂ig后,随机分为模型3 d组、模型7 d组、旋覆代赭汤低、中、高剂量组及吗丁啉组。吗丁啉组予2.7 g·kg-1的吗丁啉混悬液ig,中药低、中、高各剂量组分别予3.697,.38,14.76 g·kg-1的旋覆代赭汤ig,共给药5 d。采用放免法观测各组大鼠血液及胃窦组织中MTL,GAS,VIP的含量。结果:旋覆代赭汤各剂量组与模型组7 d组比较,可增加血液及组织中MTL含量(P<0.05或P<0.01),中、高剂量组比吗丁啉组的作用更明显;可使大鼠血液中GAS含量上升(P<0.01),中、高剂量组与吗丁啉组的作用相当;可降低血液及组织中VIP含量(P<0.05或P<0.01),高剂量组对血液中及各剂量组对组织中VIP含量的影响与吗丁啉相当。结论:旋覆代赭汤可通过调节脑肠肽在血液及组织中的含量达到促胃动力的作用。

Objective：To observe the effect for Xuanfu Daizhe Decoction（XFDZD） on motilin（MTL）,gastrin（GAS） and vasoactive intestinal peptide（VIP） content in blood and tissue in rats with low gastric motility.Method：Seventy Wistar rats were divided into normal group（10 rats） and model group（60 rats） at random.Each rat of model group was irritated stomach with liquorice decoction（10 g·kg-1）.After stomach irritation,model group were divided into model group of 3 days,model group of 7 days,low,medium,high dosage groups of XFDZD,and the group of domperidone.The stomach with low gastric motility was irritated with domperidone,and the treatment groups were treated with XFDZD at different doses.Then the content of MTL,GAS,VIP in blood and gastric antrum was tested with radioimmunopercipitation.Result：Liquorice decoction could make rat model of low gastric motility.XFDZD could make MTL,GAS content increase in rat＇s blood and organization,at the same time make VIP content reduce,campared with model groups,there were all significance difference（P0.05 or P0.01）.Conclusion：XFDZD could treat disease of gastrointestinal dismotility by regulating brain-gut peptides content in rat＇s blood or gastric antrum.