消癌解毒方体内抑瘤作用机制研究

Study on in vivo Anti-tumor Mechanism of Xiaoai Jiedu Prescription

目的:探讨消癌解毒方体内抗肿瘤作用的可能机制。方法:对荷瘤小鼠(H22肿瘤细胞株)用消癌解毒方煎剂剂量灌胃,观察光镜及电镜下肿瘤组织、细胞的形态,测定基质金属蛋白酶(MMP-2)含量。结果:消癌解毒方中剂量组凋亡细胞数量最大,并且所有消癌解毒方组中凋亡细胞除出现凋亡细胞一般的形态学改变,胞浆中可见大量吞饮空泡;消癌解毒方低剂量组外周血MMP-2水平显著降低(P<0.05),有统计学意义。结论:消癌解毒方对肿瘤抑制作用可能与其诱导肿瘤细胞凋亡,抑制MMP-2活性有关。

Objective：To study the anti-tumor mechanism of Xiaoai Jiedu prescription in vivo.Method：The mice bearing tumors（estabilished by subcutaneous inoculation of H22 cells）were fed with Xiaoai Jiedu prescription.Morphology of the tissue of tumor under light microscope and tumor cells under electron microscope were observed.The matrix metall-oproteinase（MMP-2） were assayed.Result：The medium dose group of Xiaoai Jiedu prescription has the largest amount of apoptosis.There were many pinocytosises except the general morphological change in each Xiaoai Jiedu prescription group.The activity of MMP-2 had significantly decreased in the low-dose group of Xiaoai Jiedu prescription（P0.05）.Conclusion：Xiaoai Jiedu prescription has the anti-tumor effect in vivo possibly through the mechanism of inducing tumor cell apoptosis and inhibitting the activity of MMP-2.