血吸虫抗原刺激小鼠骨髓来源的树突状细胞与DC2．4细胞的表型比较

Phenotype comparison between bone marrow derived dendritic cell and DC2.4 cell stimulated with antigen from Schistosoma japonicum

目的研究比较小鼠树突状细胞DC2．4和骨髓来源树突状细胞（bone marrow derived dendritic cell，BMDC）经血吸虫抗原谷胱甘肽转移酶（GST）刺激后表面分子的表达异同。方法骨髓来源的细胞经白介素4（interleukin4，IL-4）、粒细胞一巨噬细胞集落刺激因子（granulocyte—macrophage colony stimulating factor，GM—CSF）诱导培养，获得树突状细胞。常规方法培养DC2．4。体外用日本血吸虫抗原GST刺激前述两种细胞，以PBS和脂多糖（lipopolysaccharide，LPS）作对照，流式细胞仪检测细胞表面分子CD40、CD80、CD86的平均荧光强度，并进行统计学分析。结果日本血吸虫抗原GST刺激BMDC后，表面分子CD40、CD80、CD86的平均荧光强度依次为100．39、42．38、170．83，与PBS对照组比较，CD40无明显变化，而CD80、CD86表达上调（P〈0．01）；GST刺激DC2．4后，细胞表面分子CD40、CD80、CD86的平均荧光强度依次为23．73、72．13、59．58，与PBS对照组比较，CD40和CD86表达上调（P〈0．01），而CD80变化不明显。结论DC2．4与BMDC经日本血吸虫抗原刺激后表面分子的表达变化不同。

Objective To compare the phenotypes of bone marrow derived dendritic cell（BMDC） and DC2.4 cell stimulated with GST from Schistosorna japonicum. Methods Bone marrow cells were cultured in the presence of IL-4 and GM-CSF to induce dendritic ceils. DC2.4 cells were cultured as routine. Both cells were stimulated with GST and the expressions of CD40, CD80 and CD86 on the cells＇ surface were analyzed by FACS, using PBS and lipopolysaccharide as controls. Results After stimulating with GST, the means of fluorescence intensity （MFI） for CD40, CD80 and CD86 on BMDC surface were 100.39, 42.38 and 170.83, respectively. Compared with PBS control, the MFI of CD80 and CD86 on BMDC, but not CD40, enhanced significantly. The MFIs of CD40, CD80 and CD86 on DC2.4 loaded by GST were 23.73,72.13 and 59.58 respectively. Compared with PBS control, the expressions of CD40 and CD86 enhanced significantly after schistosome antigen stimulation. Conclusion The expressions of cell surface molecules after sehistosome antigen GST stimulation were different between BMDC and DC2.4.