摘要
目的观察内源性大麻素(AEA)对人肝癌高转移细胞MHCC97H细胞生长、黏附、侵袭性等的抑制作用。方法不同浓度AEA(100、50、0μmol/L)作用细胞后,应用体外细胞-基质黏附实验方法,检测细胞对Fibronectin黏附能力的改变;应用体外细胞趋化实验和侵袭实验,检测细胞的运动功能及对人工基底膜的侵袭能力;应用噻唑蓝(MTY)比色法检测细胞生长抑制率。结果(1)不同浓度的AEA(100、50、0μmol/L)作用细胞3、6、12h后,细胞黏附抑制率分别为:3h(24.4%、20.6%和20.8%)、6h(23.6%、21.3%和19,6%)及12h(25.2%、24.1%和22,8%),组间及组内比较差异无统计学意义(P〉0.05)。(2)100μmol/L的AEA作用细胞48h后,平均每个视野的透膜细胞数为(13.00±4.30)个,与对照组(15.70±3.30)个比较,差异无统计学意义(P〉0.05);平均每个视野的侵袭细胞数为(4.10±1.65)个,与对照组(3.40±1.24)个比较,差异无统计学意义(P〉0.05)。(3)不同浓度的AEA(100、50、0μmoL/L)作用细胞24h后,细胞生长抑制率分别为(39.30±9.17)%、(35.60±8.25)%和(28.70±0.04)%,组间比较差异无统计学意义(P〉0.05)。结论AEA对体外生长的人肝癌细胞MHCC97H的生长、黏附和侵袭性等方面无明显抑制作用。
Objective To evaluate the inhibitory effects of anandamide (AEA) on proliferation and adhesion and invasion in human hepatocellular carcinoma cell line MHCC97H. Methods Mmethyl thiazol tetrazolium (MTr) method, cell-matrix adhesion assay, and Transwell invasion chamber and matriger were used to analyze the effect of AEA on proliferation ability, adherence ability, and migration, chemotaxis and invasion of MHCC97H cells, respectively. Results After MHCC97H cells were treated with AEA at the different concentrations (100, 50, 0 μmol/L) for 3, 6 and 12 h, the inhibition ratio of cell adhesion was 24.4%, 20. 6% and 20. 8% for 3 h, 23.6%, 21.3% and 19.6% for 6 h, and 25.2%, 24. 1% and 22. 8% for 12 h, respectively (P 〉0. 05). After MHCC97H cells were treated with AEA (100μmol/L) for 24 h, the migration, adhesion and invasion was not markedly inhibited (P 〉 0. 05). Conclusion AEA did not show inhibition effects on adhesion, invasiveness and proliferation of MHCC97H cells.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2011年第6期917-919,共3页
Chinese Journal of Experimental Surgery
基金
基金项目:教育部留学回国人员科研启动基金资助项目(200314)
关键词
癌
肝细胞
内源性大麻素
黏附
侵袭
细胞增殖
Careinoma,hepatoeellular
Anandamide
Adhesion
Invasion
Cell proliferation