SLC22A18基因表达对胶质瘤细胞生物学特性的影响

Influence of SLC22A18 gene expression on the biological behaviors of glioma cells

目的观察SLC22A18基因表达对人胶质瘤U251细胞生物学特性的影响。方法U251细胞分别转染peDNA3．SLC22A18和peDNA3表达质粒后，筛选3个表达不同水平SLC22A18的U251克隆和2个不表达SLC22A18的U251克隆，研究SLC22A18蛋白表达水平对U251细胞黏附、迁移及聚集能力的影响。结果与不表达SLC22A18的U251细胞比较，表达SLC22A18的U251细胞在附着后1h和4h时的细胞黏附能力均显著增强，分别平均增强65．7％和26．5％（P〈0．01），而其细胞过河实验显示过河时间平均延长67．8％（P〈0．05），并且细胞迁移能力平均下降71．5％（P〈0．01）。同时，SLC22A18表达诱导显著的同源细胞聚集，聚集指数平均下降53．2％（P〈0．01）。结论SLC22A18蛋白表达显著抑制人胶质瘤U251细胞的恶性生物学特性。

Objective To study the effect of SLC22A18 gene expression on the biological behaviors of human glioma U251 cells. Methods Three U251 clones with different expression levels of SLC22A18 and two U251 clones without SLC22A18 expression after transfecting U251 cells with peDNA3. SLC22A18 and peDNA3 using lipofectin were chosen to study the effects of SLC22A18 gene expression on cell adhesion, motility and aggregation. Results Compared to the non-SLC22A18-expressing U251 cells, SLC22A18-ex- presing U251 cells indicated enhanced cell adhesion, and average 65.7% and 26. 5% increases in cell ad- hesion at 1 st and 4th h of the culture were observed （P 〈 0. 01 ）, but the crossing-river time of SLC22A18- expresing U251 cells was averagely prolonged by 67. 8% （P 〈 0. 05 ） , and their motility was decreased by 71.5% （P〈 0. 01 ）. At the same time, SLC22A18-expressing U251 cells demonstrated signifieantly homophilic aggregation, and aggregation index was decreased by 53.2% compared to the non-SLC22A18- expressing U251 cells （P 〈 0. 01 ）. Conclusion SLC22A18 gene expression significantly suppresses the malignant biological behaviors of U251 glioma cells.