菩人丹超微粉对糖尿病大鼠视网膜VEGF蛋白表达的影响

Effect of Purendan Ultrafine Powder on the Expression of Vascular Endothelial Growth Factor in Retina of Diabetic Rats

目的:研究菩人丹超微粉对糖尿病大鼠视网膜中血管内皮生长因子(Vascular endothelial growth factor,VEGF)蛋白表达的影响。方法:采用链脲佐菌素(Streptozocin,STZ)注入大鼠腹腔复制糖尿病大鼠模型。实验大鼠随机分为正常、模型和菩人丹超微粉组,在ig给药5个月后处死大鼠取其视网膜,测定VEGF蛋白的表达情况。ig给药1、3、5个月后分别测定大鼠血糖值。结果:正常组大鼠VEGF蛋白表达均为阴性改变,模型组呈强阳性改变,菩人丹超微粉组大鼠呈弱阳性改变,有显著性差异(P<0.01)。ig给药5个月后,模型组血糖与正常组比较显著升高,菩人丹超微粉组血糖与模型组比较显著降低(P<0.01)。结论:菩人丹超微粉可以有效抑制糖尿病大鼠视网膜中VEGF蛋白的表达,降低血糖值,从而起到延缓或治疗糖尿病视网膜病变的作用。

OBJECTIVE：To study the effect of Purendan ultrafine powder on the expression of vascular endothelial growth factor（VEGF） in retina of diabetic rats by observing the protein expression of retina of rats.METHODS：Diabetic rat models were established by intraperitoneal injection of streptozotocin（STZ）.The experimental rats were divided into normal group,model group and Purendan ultrafine powder group,and were killed in 5 months to study the expression of VGEF.The level of blood glucose in rats were determined after 1,3,5,months of ig administration.RESULTS：Negative results were obtained in normal group,weakly positive results were obtained in Purendan ultrafine powder group,and strongly positive results were obtained in model group.There were significant difference among those groups（P0.01）.After 5 months ig administration,the level of blood glucose in model group increased significantly,compared with normal group.The level of blood glucose in Purendan ultrafine powder group decreased significantly,compared with model group（P0.01）.CONCLUSION：Purendan ultrafine powder may relieve or treat diabetic retinopathy by actively suppressing the expression of VEGF and decreasing the level of blood glucose in retina of diabetic rats.