红花注射液对大鼠周围神经缺血再灌注损伤的保护作用及其机制研究

Protective Effects of Carthamus tinctorius Injection on Peripheral Nerve Ischemia-reperfusion Injury in Rats and Its mechanism

目的:研究红花注射液对大鼠周围神经缺血再灌注(IR)损伤的保护作用及其机制。方法:实验分为6组,即对照(等容生理盐水)、模型(等容生理盐水)、甘露醇(5mL.kg-1)和红花注射液高、中、低剂量组(32、16、8mL.kg-1),iv给药。阻断髂总动脉、髂内动脉和髂外动脉6h,复制周围神经IR模型。观察大鼠肢体功能,进行神经电生理检查,并检测坐骨神经组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、钙离子和肿瘤坏死因子-α(TNF-α)的水平。结果:与模型组比较,红花注射液高、中剂量组肢体功能评分和MDA水平显著降低,SOD活性显著升高(P<0.01或P<0.05);红花注射液高剂量组TNF-α和潜伏期显著下降,传导速度和波幅显著增加(P<0.01或P<0.05)。结论:红花注射液对周围神经IR损伤具有保护作用,其作用机制可能与抑制氧化应激、钙超载和炎症反应有关。

OBJECTIVE：To study the protective effects of Carthamus tinctorius injection on peripheral nerve ischemia-reperfusion（IR） injury in rats and its mechanism.METHODS：The test were randomly divided into six groups,i.e.control group（constant volume normal saline）,model group（constant volume normal saline）,mannite group（5 mL·kg-1）,C.tinctorius injection high-dose,medium-dose and low-dose groups（32,16,8 mL·kg-1） with intravenous administration.Peripheral nerve IR model was established by clamping common iliac artery,internal iliac artery and external iliac artery for 6 h.The limb function and neural electrophysiology of rats were tested.The levels of super oxide dismutase（SOD）,malonaldehyde（MDA）,calcium and tumor necrosis factor-α（TNF-α） in nervi ischiadicus tissue were detected.RESULTS：Compared with model group,the limb function and MDA of C.tinctorius injection medium-dose and high-dose groups were decreased significantly while the activity of SOD increased significantly（P0.01,P0.05）;In C.tinctorius injection high-dose group,TNF-α and latent period were decreased significantly,but conduction velocity and amplitude were increased obviously（P0.01,P0.05）.CONCLUSION：C.tinctorius injection has a protective effect for peripheral nerve IR injury in experimental rat,which may be related to the inhibition of oxidative stress,calcium overloading and inflammation.