摘要
目的:研究红花注射液对大鼠周围神经缺血再灌注(IR)损伤的保护作用及其机制。方法:实验分为6组,即对照(等容生理盐水)、模型(等容生理盐水)、甘露醇(5mL.kg-1)和红花注射液高、中、低剂量组(32、16、8mL.kg-1),iv给药。阻断髂总动脉、髂内动脉和髂外动脉6h,复制周围神经IR模型。观察大鼠肢体功能,进行神经电生理检查,并检测坐骨神经组织中超氧化物歧化酶(SOD)、丙二醛(MDA)、钙离子和肿瘤坏死因子-α(TNF-α)的水平。结果:与模型组比较,红花注射液高、中剂量组肢体功能评分和MDA水平显著降低,SOD活性显著升高(P<0.01或P<0.05);红花注射液高剂量组TNF-α和潜伏期显著下降,传导速度和波幅显著增加(P<0.01或P<0.05)。结论:红花注射液对周围神经IR损伤具有保护作用,其作用机制可能与抑制氧化应激、钙超载和炎症反应有关。
OBJECTIVE:To study the protective effects of Carthamus tinctorius injection on peripheral nerve ischemia-reperfusion(IR) injury in rats and its mechanism.METHODS:The test were randomly divided into six groups,i.e.control group(constant volume normal saline),model group(constant volume normal saline),mannite group(5 mL·kg-1),C.tinctorius injection high-dose,medium-dose and low-dose groups(32,16,8 mL·kg-1) with intravenous administration.Peripheral nerve IR model was established by clamping common iliac artery,internal iliac artery and external iliac artery for 6 h.The limb function and neural electrophysiology of rats were tested.The levels of super oxide dismutase(SOD),malonaldehyde(MDA),calcium and tumor necrosis factor-α(TNF-α) in nervi ischiadicus tissue were detected.RESULTS:Compared with model group,the limb function and MDA of C.tinctorius injection medium-dose and high-dose groups were decreased significantly while the activity of SOD increased significantly(P0.01,P0.05);In C.tinctorius injection high-dose group,TNF-α and latent period were decreased significantly,but conduction velocity and amplitude were increased obviously(P0.01,P0.05).CONCLUSION:C.tinctorius injection has a protective effect for peripheral nerve IR injury in experimental rat,which may be related to the inhibition of oxidative stress,calcium overloading and inflammation.
出处
《中国药房》
CAS
CSCD
北大核心
2011年第23期2139-2141,共3页
China Pharmacy
关键词
红花
周围神经
缺血再灌注
氧化应激
钙超载
炎症
Carthamus tinctorius
Peripheral nerve
Ischemia reperfusion
Oxidative stress
Calcium overloading
Inflammation